Cyclic Nucleotide Signaling in Plants

Molecular Methods for the Study of Signal Transduction in Plants.- Recombinant Expression and Functional Testing of Candidate Adenylate Cyclase Domains.- Quantification of Cyclic Dinucleotides by Reversed-Phase LC-MS/MS.- Determination of ADP-ribosyl cyclase activity, cyclic ADP-ribose and Nicotinamide adenine dinucleotide phosphate in Tissue Extracts.- In vivo imaging of cGMP in plants.- Characterization of Heterologously Expressed Transporter Genes by Patch- and Voltage-Clamp Methods: Application to Cyclic Nucleotide-Dependent Responses.- Non-invasive Microelectrode Ion Flux Estimation Technique (MIFE) for the study of the regulation of root membrane transport by cyclic nucleotides.- Calcium imaging of the cyclic nucleotide response.- Identification and Quantification of Signal Molecule-dependent Protein Phosphorylation.- Comparative Gel-based (Phospho)proteomics in Response to Signaling Molecules.- An Affinity Pull-down Approach to Identify the Plant Cyclic Nucleotide Interactome.- Structural and Functional Characterization of Receptor Kinases with Nucleotide Cyclase Activit.- Computational Identification of Candidate Nucleotide Cyclases in Higher Plants.- Identification of Cyclic Nucleotide Gated Channels Using Regular Expressions.- Inferring biological functions of guanylyl cyclases with computational methods.- Identification and Characterization of Cyclic Nucleotide Phosphodiesterases.- Detection of reactive oxygen species down-stream of cyclic nucleotide signals in plants.- Measurement of nitric oxide in plant tissue using difluorofluorescein and oxyhaemoglobin.- Infra-red Gas Analysis (IRGA) Technique for the Study of the Regulation of Photosynthetic Responses.