RNA-RNA Interactions

Methods and Protocols

Frank J. Schmidt (Herausgeber)

Buch | Softcover
219 Seiten
2016 | Softcover reprint of the original 1st ed. 2015
Humana Press Inc. (Verlag)
978-1-4939-4161-2 (ISBN)

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RNA-RNA Interactions -
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In this volume expert researchers in the field detail many of the methods which are now commonly used to study RNA. These methods are presented as a guidebook to scientists who are experienced with RNA research and want to brush up on a new technique. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Thorough and intuitive, RNA-RNA Interactions: Methods and Protocols guides scientists investigating biological systems and studying RNA.

Identifying RNA Recombination Events and Non-covalent RNA-RNA Interactions with the Molecular Colony Technique.- RNA-directed Recombination of RNA in Vitro.- RNA-RNA Selex.- Identification of Antisense RNA S0tem-loops that Inhibit RNA-protein Interactions using a Bacterial Reporter System.- Trans-activation of Large Ribozymes.- Native Purification and Labeling of RNA for Single Molecule Fluorescence Studies.- Single Molecule Studies of RNA-RNA Interactions.- Modification Interference Analysis of the Ribosome.- Assessing Intermolecular RNA:RNA Interactions Within a Ribonucleoprotein Complex Using Heavy Metal Cleavage Mapping.- Monitoring RNA-RNA Interactions With Electrophoretic Mobility Shift Assays: Analysis Of Trna Binding To The T Box Riboswitch Antiterminator RNA.- Electrophoretic Mobility Shift Assay of RNA-RNA Complexes.- Structural Studies of a Double-stranded RNA from Trypanosome RNA Editing by Small Angle X-ray Scattering.- Fusion RNAs in Crystallographic Studies of Double-stranded RNA from Trypanosome RNA Editing.

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology ; 1240
Zusatzinfo 6 Illustrations, color; 31 Illustrations, black and white; X, 219 p. 37 illus., 6 illus. in color.
Verlagsort Totowa, NJ
Sprache englisch
Maße 178 x 254 mm
Themenwelt Medizin / Pharmazie Medizinische Fachgebiete
Studium 2. Studienabschnitt (Klinik) Humangenetik
Naturwissenschaften Biologie Genetik / Molekularbiologie
Schlagworte bacterial cells • catalyze chemical reactions • gene expression • peptide bond • regulatory RNAs • RNA • RNA-RNA interactions • small RNAs • synthesis
ISBN-10 1-4939-4161-5 / 1493941615
ISBN-13 978-1-4939-4161-2 / 9781493941612
Zustand Neuware
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