Hypertension

Large Scale Transcriptome Analysis.- Methods to Assess Genetic Risk Prediction.- Microarray Analysis of Hypertension.- Tissue Proteomics in Vascular Disease.- Urine Metabolomics in Hypertension Research.- Systems Biology Approach in Hypertension Research.- Measurement of Angiotensin Peptides: HPLC, RIA.- Measurement of Angiotensin Converting Enzyme 2 Activity in Biological Fluid (ACE2).- Determining the Enzymatic Activity of Angiotensin-Converting Enzyme 2 (ACE2) in Brain Tissue and Cerebrospinal Fluid Using a Quenched Fluorescent Substrate.- Measurement of Cardiac Angiotensin II by Immunoassays, HPLC-chip/Mass Spectrometry and Functional Assays.- Analysis of the Aldosterone Synthase (CYP11B2) and 11b-Hydroxylase (CYP11B1) Genes.- Dopaminergic Immunofluorescence Studies in Kidney Tissue.- Techniques for the Evaluation of the Genetic Expression, Intracellular Storage and Secretion of Polypeptide Hormones with SpecialReference to the Natriuretic Peptides (Nps).- Intracellular Free Calcium Measurement Using Confocal Imaging.- Measuring T-type Calcium Channel Currents in Isolated Vascular Smooth Muscle Cells.- In Vitro Analysis of Hypertensive Signal Transduction:  Kinase Activation, Kinase Manipulation, and Physiologic Outputs.- In vitro and in vivo Approaches to Assess Rho-kinase Activity.- NADPH Oxidases and Measurement of Reactive Oxygen Species.- Measurement of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation.- Assessment of Caveolae/Lipid Rafts in Isolated Cells.- Isolation and Characterization of Circulating Microparticles by Flow Cytometry.- Isolation of Mature Adipocytes from White Adipose Tissue and Gene Expression Studies by Real-Time Quantitative RT-PCR.- Isolation and Differentiation of Murine Macrophages.- Isolation and Differentiation of Human Macrophages.- Isolation of Immune Cells for Adoptive Transfer.- Isolation and Culture of Endothelial Cells from Large Vessels.- Isolation and Culture of Vascular Smooth Muscle Cells from Small and Large Vessels.- Evaluation of Endothelial Dysfunction in vivo.- Vascular Reactive of Isolated Aorta To Study the Angiotensin-(1-7) Actions.- Generation of a Mouse Model with Smooth Muscle Cell Specific Loss of the Expression of< PPARγ.- Renal Delivery of Anti-microRNA Oligonucleotides in Rats.- In Vivo Analysis of Hypertension: Induction of Hypertension, In Vivo Kinase Manipulation And Assessment Of Physiologic Outputs.