Diagnostic Molecular Biology

Chang-Hui Shen, PhD, is a Professor of Biology and Biochemistry at the College of Staten Island and the Graduate Center, City University of New York. He earned his B.S. and M.S. at the National Chung-Hsing University, Taiwan, and his Ph.D. at the University of Edinburgh, UK. He was a visiting fellow of the National Institutes of Health before joining the City University of New York. Dr. Shen’s research focuses on the mechanism of gene expression, with an emphasis on epigenetic regulation in gene activation. His lab utilizes the breadth of current and advanced techniques in molecular biology and biochemistry. He has published dozens of articles throughout his career, and his research has been supported by several agencies, including NATO, NIH, and NSF.

Part I1. Nucleic Acids: DNA and RNA2. Nucleic Acid-Based Cellular Activities3. Gene Expression: Transcription of the Genetic Code4. Gene Expression: Translation of the Genetic Code5. The Genome

Part II6. Extraction and Purification of Nucleic Acids and Proteins7. Detection and Analysis of Nucleic Acids8. Quantification and Analysis of Proteins9. Amplification of Nucleic Acids10. Characterization of Nucleic Acids and Proteins11. Techniques in Sequencing12. Genome and Transcriptome Analysis

Part III13. Molecular Diagnosis of Chromosomal Disorders14. Molecular Diagnosis of Mutation and Inherited Diseases15. Molecular Diagnosis of Infectious Diseases16. Guidance for Molecular Clinical Laboratory