Histone Deacetylases

A Sensitive and Flexible Assay for Determining Histone Deacetylase 1 (HDAC1) Activity.- Detection of Sumo Modification of Endogenous Histone Deacetylase 2 (HDAC2) in Mammalian Cells.- Analysis of Epigenetic Regulation of Hypoxia-Induced Epithelial-Mesenchymal Transition in Cancer Cells by Quantitative Chromatin Immunoprecipitation of Histone Deacetylase 3 (HDAC3).- Molecular and Functional Characterization of Histone Deacetylase 4 (HDAC4).- Approaches for Studying the Subcellular Localization, Interactions, and Regulation of Histone Deacetylase 5 (HDAC5).- Analysis of Expression and Functions of Histone Deacetylase 6 (HDAC 6).- Analysis of Histone Deacetylase 7 (HDAC7) Alternative Splicing and Its Role in Embryonic Stem Cell Differentiation toward Smooth Muscle Lineage.- Large-Scale Overproduction and Purification of Recombinant Histone Deacetylase 8 (HDAC8) from the Human-Pathogenic Flatworm Schistosoma mansoni.- Visualization of Histone Deacetylase9 (HDAC9) Spatiotemporal Subcellular Localization in Primary Neuron Cultures.- Expression and Function of Histone Deacetylase 10 (HDAC10) in B Cell Malignancies.- Functional Analysis of Histone Deacetylase 11 (HDAC11).- Sirtuin1 (SIRT1) in the Acetylation of Downstream Target Proteins.- Protocols for Cloning, Expression, and Functional Analysis of Sirtuin2 (SIRT2).- Cloning and Characterization of Sirtuin3 (SIRT3).- Identification of Sirtuin4 (SIRT4) Protein Interactions: Uncovering Candidate Acyl-Modified Mitochondrial Substrates and Enzymatic Regulators.- Generation and Purification of Catalytically Active Recombinant Sirtuin5 (SIRT5) Protein.- Sirtuin6 (SIRT6) Activity Assays.- Molecular, Cellular, and Physiological Characterization of Sirtuin7 (SIRT7).- HDAC Inhibitors.- Assessment of the Anti-Proliferative Activity of a BET Bromodomain Inhibitor as Single Agent and in Combination in Non-Hodgkin Lymphoma Cell Lines.- Screening the Impact of Sirtuin Inhibitors on Inflammatory and Innate Immune Responses of Macrophages and in a Mouse Model of Endotoxic Shock.