Methods in Cilia and Flagella

Wallace Marshall is an electrical engineer by training, who became interested in biology out of a desire to understand how cells solve engineering problems, such as determining the size of organelles. He received his Ph.D. at UCSF with John Sedat, where he studied the diffusional of motion of interphase chromatin using live cell imaging and computational image analysis. He then trained as a postdoc with Joel Rosenbaum at Yale, where he began studying the mechanisms regulating the length of cilia and flagella. He is now Profess of Biochemistry at UCSF, where he lab continues to study the assembly and length regulation of cilia and flagella, as well as the mechanisms that regulate the size of other organelles. His work takes advantage of an integrated combination of methods including genetics, microscopy, and computational modeling, as well as a wide variety of model organisms including Chlamydomonas reinhardtii, Stentor coeruleus, yeast, flatworms, and mammalian cells.

1. Imaging centrosomes and cilia in the mouse kidney Masato Hoshi, Jinzhi Wang, Sanjay Jain and Moe R. Mahjoub 2. Ependymal cell differentiation, from monociliated to multiciliated cells Nathalie Delgehyr, Alice Meunier, Marion Faucourt, Montserrat Bosch Grau, Laetitia Strehl, Carsten Janke, Nathalie Spassky 3. Observing planar cell polarity in multiciliated mouse airway epithelial cells Eszter K. Vladar, Yin Loon Lee, Tim Stearns and Jeffrey D. Axelrod 4. Imaging and analysing primary cilia in cardiac cells Nicolas Diguet, Jean-François Le Garrec, Tommaso Lucchesi and Sigolène M. Meilhac 5. Cilia in photoreceptors Linjing Li, Manisha Anand, Kollu Nageswara Rao and Hemant Khanna 6. Analysis of primary cilia in the developing mouse brain Judith TML Paridaen, Wieland B Huttner and Michaela Wilsch-Bräuninger 7. In vivo investigation of cilia structure and function using Xenopus Eric R. Brooks and John B. Wallingford 8. A quantitative approach to study endothelial cilia bending stiffness during blood flow mechanodetection in-vivo Francesco Boselli, Jacky G. Goetz, Gilles Charvin, Julien Vermot 9. Quantitative description of fluid flows produced by left-right cilia in zebrafish Craig Fox, M. Lisa Manning and Jeffrey D. Amack 10. Efficient live fluorescence imaging of intraflagellar transport in mammalian primary cilia Hiroaki Ishikawa and Wallace F. Marshall 11. Analysis of soluble protein entry into primary cilia using semi-permeabilized cells David K. Breslow and Maxence V. Nachury 12. Methods for imaging individual cilia in living echinoid embryos Robert L. Morris, Hans W. Pope, Adam N. Sholi, Leah M. Williams, Chelsea R. Ettinger, Gwendolyn M. Beacham, Tatsushi Shintaku, Zachary D. Abbott, Elyse M. Doherty 13. The planarian Schmidtea mediterranea as a model for studying motile cilia and multiciliated cells Cyril Basquin, Anne-Marie Orfila and Juliette Azimzadeh 14. Quantitative analysis of flagellar proteins in Drosophila sperm tails Teresa Mendes Maia, Perrine Paul-Gilloteaux, Renata Basto 15. Imaging cilia in Drosophila melanogaster Jennifer Vieillard, Jean-Luc Duteyrat, Elisabeth Cortier, Bénédicte Durand 16. Studying G-protein-coupled receptors: Immunoblotting, immunoprecipitation, phosphorylation, surface labeling, and cross-linking protocols Kasturi Pal, Hemant Badgandi, and Saikat Mukhopadhyay 17. Image analysis of Caenorhabditis elegans ciliary transition zone structure, ultrastructure, molecular composition and function Anna A. W. M. Sanders, Julie Kennedy and Oliver E. Blacque 18. Genetic and genomic approaches to identify genes involved in flagellar assembly in Chlamydomonas reinhardtii Huawen Lin and Susan K. Dutcher 19. Axonemal motility in Chlamydomonas Ken-ichi Wakabayashi and Ritsu Kamiya