Human Cytomegaloviruses

History of the Molecular Biology of Cytomegalovirus.- Overview of Human Cytomegalovirus Pathogenesis.- Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies.- Use of Diploid Human Fibroblasts as a Model System to Culture/Grow, Study and Understand Human Cytomegalovirus Infection.- Use of Recombinant Approaches to Construct Human Cytomegalovirus Mutants.- The Use of Primary Human Cells (Fibroblasts, Monocytes and Others) to Assess Human Cytomegalovirus Function.- Human Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation.- Analysis of Cytomegalovirus Binding/Entry-Mediated Events.- Use of 5-Ethynyl-2’-Deoxyuridine Labelling and Flow Cytometry to Study Cell Cycle-Dependent Regulation of Human Cytomegalovirus Gene Expression.- Methods for Studying the Function of Cytomegalovirus GPCRs.- Methods for the Detection of Cytomegalovirus in Glioblastoma Cells and Tissues.- Methods to Study the Nucleocytoplasmic Transport of Macromolecules with Respect to Their Impact on the Regulation of Human Cytomegalovirus Gene Expression.- Fluorescence-Based Laser Capture Microscopy Technology Facilitates Identification of Critical In Vivo Cytomegalovirus Transcriptional Programs.- Techniques for Characterizing Cytomegalovirus-Encoded miRNAs.- What We Have Learned From Animal Models of HCMV.- Rodent Models of Congenital Cytomegalovirus Infection.- Recent Approaches and Strategies in the Generation of Anti-HumanCytomegalovirus Vaccines.- Approaches for the Generation of New Anti-Cytomegalovirus Agents:  Identification of Protein-Protein Interaction Inhibitors and Compounds Against the HCMV IE2 Protein.