Isotope labeling in Biomolecular NMR (eBook)
VIII, 219 Seiten
Springer Netherland (Verlag)
978-94-007-4954-2 (ISBN)
NMR spectroscopy has undergone a revolution in recent years with the advent of several new methods overcoming the problems of sensitivity and resolution. Recent developments in biotechnology have made it easier and economical to introduce 13C, 15N and 2H into proteins and nucleic acids. At the same time, there has been an explosion in the number of NMR experiments that utilize such isotope labeled samples. Thus, a combination of isotopic labeling and multidimensional, multinuclear NMR has opened up new avenues for structural studies of proteins, nucleic acids and their complexes.
This book will focus on recent developments in isotope labeling methods for structural studies of small molecules, peptides, proteins and nucleic acids. The aim of the book is to serve as a compendium of isotope labeling for the biomolecular NMR community providing comprehensive coverage of the existing methods and latest developments along with protocols and practical hints on the various experimental aspects. The book will cover a wide range of topics in isotope labeling under one title including emerging areas of metabolonomics and solid state NMR.
NMR spectroscopy has undergone a revolution in recent years with the advent of several new methods overcoming the problems of sensitivity and resolution. Recent developments in biotechnology have made it easier and economical to introduce 13C, 15N and 2H into proteins and nucleic acids. At the same time, there has been an explosion in the number of NMR experiments that utilize such isotope labeled samples. Thus, a combination of isotopic labeling and multidimensional, multinuclear NMR has opened up new avenues for structural studies of proteins, nucleic acids and their complexes. This book will focus on recent developments in isotope labeling methods for structural studies of small molecules, peptides, proteins and nucleic acids. The aim of the book is to serve as a compendium of isotope labeling for the biomolecular NMR community providing comprehensive coverage of the existing methods and latest developments along with protocols and practical hints on the various experimental aspects. The book will cover a wide range of topics in isotope labeling under one title including emerging areas of metabolonomics and solid state NMR.
PROTEINS1. Isotope labeling methods for large systems; Patrik Lundström, Alexandra Ahlner and Annica Theresia Blissing2. Segmental labeling to study multidomain proteins; Jing Xue, David S. Burz and Alexander Shekhtman3. Isotope Labeling for Solution and Solid-State NMR Spectroscopy of Membrane Proteins; Raffaello Verardi, Nathaniel J. Traaseth, Larry R. Masterson, Vitaly V. Vostrikov and Gianluigi Veglia 4. Isotope labeling methods for relaxation measurements; Patrik Lundström, Alexandra Ahlner and Annica TheresiaBlissing5. Stereo-array isotope labeling method for studying protein structure and dynamics; Yohei Miyanoiri, Mitsuhiro Takeda and Masatsune Kainosho 6. Amino acid selective labeling and unlabeling for protein resonance assignments; Garima Jaipuria, B. Krishnarjuna, Somnath Mondal, Abhinav Dubey, and Hanudatta S. Atreya NUCLEIC ACIDS 7. Isotope labeling and segmental labeling of larger RNAs for NMR structural studies; Olivier Duss, Peter J. Lukavsky, and Frédéric H.-T. AllainMETABOLOMICS 8. Isotope Enhanced Approaches in Metabolomics; G. A. Nagana Gowda, N. Shanaiahand and Daniel RafteryEXPRESSION SYSTEMS 9. Cell-free protein synthesis using E. coli cell extract for NMR studies; Mitsuhiro Takeda and Masatsune Kainosho10. Isotope labeling in insect cells; Alvar D. Gossert and Wolfgang Jahnke11. Mammalian Expression of Isotopically Labeled Proteins for NMR Spectroscopy; Mallika Sastry, Carole A. Bewley and Peter D. Kwong
| Erscheint lt. Verlag | 18.10.2012 |
|---|---|
| Reihe/Serie | Advances in Experimental Medicine and Biology | Advances in Experimental Medicine and Biology |
| Zusatzinfo | VIII, 219 p. 75 illus., 45 illus. in color. |
| Verlagsort | Dordrecht |
| Sprache | englisch |
| Themenwelt | Studium ► 1. Studienabschnitt (Vorklinik) ► Biochemie / Molekularbiologie |
| Naturwissenschaften ► Biologie ► Biochemie | |
| Naturwissenschaften ► Chemie ► Analytische Chemie | |
| Naturwissenschaften ► Physik / Astronomie | |
| Technik ► Umwelttechnik / Biotechnologie | |
| Schlagworte | Biomolecular NMR • Genetic Engineering • isotope labeling • Nucleic acids • structural biology |
| ISBN-10 | 94-007-4954-6 / 9400749546 |
| ISBN-13 | 978-94-007-4954-2 / 9789400749542 |
| Haben Sie eine Frage zum Produkt? |
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