Methods in DNA Amplification

General Aspects of PCR.- The Polymerase Chain Reaction and Fixed Tissues.- Impact of PCR on the Pathologist’s World.- Sensitive and Rapid Detection and Quantification of Nucleic Acids.- Polymorphic Keratins as Detected by PCR and SSCP.- Production of Antibodies of Monoclonal Specificity without the Use of Hybridoma Cell Lines.- Use of Chelex 100TM in the Extraction of Viruses from Diverse Cell-Free Clinical Samples for PCR.- Detection of Amplified Oncogenes by Differential Polymerase Chain Reaction.- Resolution of a Secondary Structure in an Unknown mRNA 5’End.- Automated Solid-Phase Sequencing of Genomic DNA Obtained from Polymerase Chain Reaction.- Alternative DNA-Amplification Methods.- Ligase-Mediated Detection Techniques.- Qualitative and Quantitative Detection of Nucleic Acids of Infectious Agents by NASBA.- Application of NASBA to the Detection of Listeria Monocytogenes.- PCR and Virological Problems.- Confirmation of Hepatitis C Virus Carrier State by Polymerase Chain Reaction in Cases with Nondiagnostic Serologies.- Confirmation of Hepatitis C Virus Positive Seras by Polymerase Chain Reaction.- Specific Detection and Rapid Identification of Human Enteroviruses by PCR Amplification.- Detection of Herpes Simplex Virus Using Polymerase Chain Reaction.- Detection of Human Cytomegalovirus (HCMV)-DNA from Granulocytes and Peripheral Blood Mononuclear Cells (PBMNC) of Immunosuppressed Patients by Nested PCR: Lack of Correlation to Isolation of Infectious Virus.- Timing of Mother-to Child Transmission of HIV-1 as Determinated by Nested Polymerase Chain Reaction - A Cohort Study in Kigali, Rwanda.- One-Day Detection of Enteroviruses in Clinical Specimens by Magnetic Bead Extraction of Viral RNA and Nested Polymerase Chain Reaction.- Monitoring of Poliovirus StrainsCirculating in the Environment and Their Intertypic and Intratypic Differentiation by RFLP Analysis of a PCR Product Derived from the 5’Non-coding Region.- Detection of Adenoviruses in Blood by Polymerase Chain Reaction.- Detection of the HPV 16 E2 Gene in Nonkeratinizing Squamous Cervical Carcinomas by PCR.- PCR in the Field of Bacterial and Fungal Problems.- Identification of Toxoplasma-DNA by Polymerase Chain Reaction in Peripheral Blood Leukocytes of Patients with Suspected Toxoplasmosis.- Detection of Mycobacterium Tuberculosis in Clinical Samples by Polymerase Chain Reaction.- Tuberculosis in HIV Infected Patients Detected by PCR: A Comparison with Clinical Data.- Detection of Borrelia Burgdorferi in Human Skin Biopsies by a Nested Polymerase Chain Reaction.- The Application of PCR Fingerprinting to Epidemiological Analysis of Bacterial and Fungal Pathogens.- Contributors.- Suppliers of Special Items.