Basic Techniques in Molecular Biology

1 General Aspects of DNA Isolation and Purification.- 2 Preparation of Genomic DNA from Animal Cells.- 3 Preparation of Genomic DNA from Plant Cells.- 4 Preparation of Genomic DNA from Bacteria.- 5 Isolation of Plasmid DNA.- 6 Isolation and Purification of RNA.- 7 Isolation of PolyA+ RNA.- 8 Agarose Gel Electrophoresis of DNA.- 9 Preparation of Probes for Hybridization.- 10 Nucleic Acid Hybridization. A Theoretical Consideration.- 11 DNA Transfer and Hybridization.- 12 Northern Transfer and Hybridization.- 13 DNA Cloning. General Consideration.- 14 DNA Cloning - Experimental Procedures.- 15 DNA Sequencing.- 16 PCR Analysis.

This laboratory manual differs from many of the numerous others currently on the market in that is explains the "Whys" as well as the "Hows" of the most commonly used molecular techniques. ... It is absolutely jam packed full of those little titbits of information that friendly Post Docs occasionally feel inclined to share with students. ... I liked this book, ... (it) is principally aimed at students ... and the emphasis is very definitely on making techniques accessible to these people. ... this particular book more than adequately fills the niche it is intended to cover.

Kate E. Plant, Univ. of Oxford, UK in Journal of Cell Science 2000, 113