Sphingolipid Metabolism and Cell Signaling, Part B
Academic Press Inc (Verlag)
978-0-12-182213-2 (ISBN)
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Section I: Methods for Analyzing Sphingolipids
[1]: Analysis of Sphingoid Bases and Sphingoid Base 1-Phosphates by High-Performance Liquid Chromatography
[2]: Enzymatic Method for Measurement of Sphingosine 1-Phosphate
[3]: Ceramide Mass Analysis by Normal-Phase High-Performance Liquid Chromatography
[4]: Quantitative Determination of Ceramide Using Diglyceride Kinase
[5]: Analysis of Sphingomyelin, Glucosylceramide, Ceramide, Sphingosine, and Sphingosine 1-Phosphate by Tandem Mass Spectrometry
[6]: Analyses of Glycosphingollpids by High-Performance Liquid Chromatography
[7]: Sphingolipid Extraction and Analysis by Thin-Layer Chromatography
[8]: Extraction and Analysis of Multiple Sphingolipids from a Single Sample
[9]: Purification of Sphingolipid Classes by Solid-Phase Extraction with Aminopropyl and Weak Cation Exchanger Cartridges
[10]: Ganglioside Analysis by High-Performance Thin-Layer Chromatography
[11]: Purification and Analysis of Gangliosides
[12]: Thin-Layer Chromatography Blotting Using Polyvinylidene Difluoride Membrane (Far-Eastern Blotting) and Its Applications
[13]: Thin-Layer Chromatography Immunostaining
[14]: Monoclonal Anti-Glycosphingolipid Antibodies
[15]: Immunolocalization of Gangliosides by Light Microscopy Using Anti-Ganglioside Antibodies
[16]: Cloud-Point Extraction of Gangliosides using Nonionic Detergent C14EO6
[17]: Analyses of Glycosphingolipids Using Clam, Mercenaria mercenaria, Ceramide Glycanase
[18]: Quantitative Analyses of Binding Affinity and Specificity for Glycolipid Receptors by Surface Plasmon Resonance
[19]: Use of Circular Dichroism for Assigning Stereochemistry of Sphingosine and Other Long-Chain Bases
[20]: Infrared Determination of Conformational Order and Phase Behavior in Ceramides and Stratum Corneum Models
[21]: Use of Nuclear Magnetic Resonance Spectroscopy in Evaluation of Ganglioside Structure, Conformation, and Dynamics
[22]: Fluorescence Quenching Assay of Sphingolipid/Phospholipid Phase Separation in Model Membranes
Section II: Methods for Analyzing Aspects of Sphingolipid Metabolism in Intact Cells
[23]: Synthesis of Fluorescent Substrates and Their Application to Study of Sphingolipid Metabolism in Vitro and in Intact Cells
[24]: Selection of Mammalian Cell Mutants in Sphingolipid Biosynthesis
[25]: Selection of Yeast Mutants in Sphingolipid Metabolism
[26]: Fluorescence-Based Selection of Gene-Corrected Hematopoietic Stem and Progenitor Cells Based on Acid Sphingomyelinase Expression
[27]: Mammalian Ganglioside Sialidases: Preparation and Activity Assays
Section III: Sphingolipid–Protein Interactions and Cellular Targets
[28]: Effects of Sphingosine and Other Sphingolipids on Protein Kinase C
[29]: Kinetic Analysis of Sphingoid Base Inhibition of Yeast Phosphatidate Phosphatase
[30]: Assays of Sphingosine-Dependent Kinase for 14-3-3 Protein
[31]: Synthesis and Use of Caged Sphingolipids
[32]: Binding of Sphingosine 1-Phosphate to Cell Surface Receptors
[33]: Use of Short-Chain Ceramides
[34]: Analysis of Ceramide-Activated Protein Phosphatases
[35]: Use of Affinity Chromatography and TID-Ceramide Photoaffinity Labeling for Detection of Ceramide-Binding Proteins
[36]: Lectin-Mediated Cell Adhesion to Immobilized Glycosphingolipids
[37]: Analysis of Glycolipid-Dependent Cell Adhesion Based on Carbohydrate–Carbohydrate Interaction
[38]: Analysis of Interactions between Glycosphingolipids and Microbial Toxins
[39]: Oxidation of Aglycone of Glycosphingolipids: Serine and Ceramide Acid Precursors for Soluble Glycoconjugates
[40]: Separation of Glycosphingolipid-Enriched Microdomains from Caveolar Membrane Characterized by Presence of Caveolin
[41]: Reconstitution of Sphingolipid–Cholesterol Plasma Membrane Mlcrodomalns for Studies of Virus-Glycolipid Interactions
[42]: Analysis of Ceramides Present in Glycosylphosphatidylinositol Anchored Proteins of Saccharomyces cerevisiae
[43]: Preparation of Functionalized Lipid Tubules for Electron Crystallography of Macromolecules
Section IV: Sphingolipid Transport and Trafficking
[44]: Applications of BODIPY–Sphingolipid Analogs to Study Lipid Traffic and Metabolism in Cells
[45]: Using Biotinylated Gangliosides to Study Their Distribution and Traffic in Cells by Immunoelectron Microscopy
[46]: Assays for Transmembrane Movement of Sphingolipids
Section V: Other Methods
[47]: Compilation of Methods Published in Previous Volumes of Methods in Enzymology
Author Index
Subject Index
Erscheint lt. Verlag | 25.10.2000 |
---|---|
Reihe/Serie | Methods in Enzymology |
Mitarbeit |
Chef-Herausgeber: John N. Abelson, Melvin I. Simon |
Verlagsort | San Diego |
Sprache | englisch |
Maße | 152 x 229 mm |
Gewicht | 1040 g |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Zellbiologie | |
ISBN-10 | 0-12-182213-3 / 0121822133 |
ISBN-13 | 978-0-12-182213-2 / 9780121822132 |
Zustand | Neuware |
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