Electron Microscopy in Microbiology (eBook)

Cover 1
Copyright Page 5
Contributors 6
Preface 8
Contents 10
I. Aspects of Morphology and Cytology of Microorganisms 12
1. Cryopreparation of Microorganisms for Electron Microscopy 12
2. Analysis of Crystalline Bacterial Surface Layers by Freeze-etching, Metal Shadowing, Negative Staining, and Ultra-thin Sectioning 40
3. Analysis of the Structure and Development of Bacterial Membranes (Outer, Cytoplasmic and Intracytoplasmic Membranes) 72
4. Analysis of Refractile (R) Bodies 90
II. Identification, Localization, and Quantitation of Low-Molecular Weight Cellular Components 102
5. Electron Probe Microanalysis of Cryosections from Cell Suspensions 102
6. Electron Spectroscopic Imaging: an Advanced Technique for Imaging and Analysis in Transmission Electron Microscopy 124
III. Identification and Localization of High-Molecular Weight Cellular Components 158
7. Immunoelectron Microscopy of Surface Antigens (Polysaccharides) of Gram-negative Bacteria using Pre- and Post-embedding Techniques 158
8. Immunoelectron Microscopic Localization of Bacterial Enzymes: Pre- and Post-embedding Labelling Techniques on Resin-embedded Samples 186
9. Localization of Macromolecular Components by Application of the Immunogold Technique on Cryo-sectioned Bacteria 222
10. Localization of Bacterial Enzymes by Electron Microscopic Cytochemistry as Demonstrated for the Polar Organelle 248
IV. Isolated Bacterial Membranes and Proteoliposomes 272
11. Electron Microscopy of Isolated Microbial Membranes 272
12. Analysis of Dimensions and Structural Organization of Proteoliposomes 294
V. Electron Microscopy at Macromolecular Dimensions 304
13. Electron Microscopic Analysis of Nucleic Acids and Nucleic Acid–Protein Complexes 304
14. Interaction of Restriction Endonucleases with DNA as Revealed by Electron Microscopy 336
15. Preparation of Two-dimensional Arrays of Soluble Proteins as Demonstrated for Bacterial D-ribulose-1,5-bisphosphate Carboxy- lase/Ox ygenase 352
16. Correlation Averaging and 3-D Reconstruction of 2-D Crystalline Membranes and Macromolecules 368
Index 426