Advanced Techniques in Biophysics (eBook)

Drs. Arrondo and Alonso are Professors in Biochemistry and Molecular Biology at the University of the Basque Country, which includes the Chair of Biophysics. Dr. Alonso has been teaching biophysics for more than 10 years and Dr. Arrondo for 5 years. Both are also members of the "Unidad de Biofisica", a joint research institute between the University of the Basque Country and the Spanish Research Council (CSIC), which they have headed for different periods.

Preface 5
Contents 7
List of Contributors 13
Chapter 1 Protein Nanocrystallization 15
Chapter 2 Differential Scanning Calorimetry of Proteins: an Overview and Some Recent Developments 41
Chapter 3 IR Reflectance - Absorbance Studies of Peptide Structure, Orientation, and Conformational Flexibility in Langmuir Films: Relevance for Models of Pulmonary Surfactant Action 63
Chapter 4 Two-Dimensional Infrared Correlation Spectroscopy 87
Chapter 5 NMR Spectroscopy of Large Biological Macromolecules in Solution 103
Chapter 6 Emerging Techniques in Fast Multidimensional NMR 143
Chapter 7 Principles and Application of Projected Multidimensional NMR Spectroscopy - G-matrix Fourier Transform NMR 161
Chapter 8 Protein Nanomechanics - as Studied by AFM Single-Molecule Force Spectroscopy 177
Chapter 9 Multiphoton-Excitation Fluorescence Microscopy and Membranes 261
Chapter 10 Three-Dimensional Electron Microscopy. The Coming of Age of a Versatile Structural Biology Technique 281
Subject Index 293