Introduction to Fluorescence

David M. Jameson is a professor in the Department of Cell and Molecular Biology at the John A. Burns School of Medicine, University of Hawaii at Manoa, having previously served there as professor and chairman of the Department of Biochemistry and Biophysics. He earned his Ph.D. in biochemistry from the University of Illinois at Urbana-Champaign, where his graduate thesis advisor was Professor Gregorio Weber. Prior to his move to the University of Hawaii, he was a postdoctoral fellow at the University of Illinois at Urbana-Champaign and the CNRS, University of Paris-South and he was an assistant professor in the Department of Pharmacology at the University of Texas Southwestern Medical Center. Dr. Jameson is the co-organizer of the International Weber Symposia on Innovative Fluorescence Methodologies in Biochemistry and Medicine, which have been held every three years since 1986 (since 1995 in Hawaii). He serves on the editorial boards of The Scientific World Journal, Analytical Biochemistry, and Methods and Applications in Fluorescence. He is a member of the advisory board for the Laboratory for Fluorescence Dynamics (supported by the National Institutes of Health) at the University of California, Irvine. He was the recipient of the 2004 Gregorio Weber Award for Excellence in Fluorescence Theory/Application.

Introduction. Absorption of Light. Instrumentation. Emission and Excitation Spectra. Polarization and Anisotropy. Time-Resolved Fluorescence. Quantum Yields and Quenching. Förster Resonance Energy Transfer. Brief Overview of Fluorescence Microscopy. Fluorophores. Intrinsic Protein Fluorescence. Appendix. Index.