Nuclear Reprogramming

Nuclear Transplantation in Xenopus.- Nuclear Transfer in Sheep.- Protocols for Nuclear Transfer in Mice.- Isolation of Stromal Stem Cells From Human Adipose Tissue.- Nuclear Reprogramming by Cell Fusion.- Polyethylene Glycol-Mediated Cell Fusion.- Epigenetic Reprogramming of Somatic Genomes by Electrofusion With Embryonic Stem Cells.- Quantification of Cell Fusion by Flow Cytometry.- Modulation of Cell Fate Using Nuclear and Cytoplasmic Extracts.- Transgenic Systems in Nuclear Reprogramming.- Using Immunofluorescence to Observe Methylation Changes in Mammalian Preimplantation Embryos.- Observing S-Phase Dynamics of Histone Modifications With Fluorescently Labeled Antibodies.- Quantitative Analysis of Telomerase Activity and Telomere Length in Domestic Animal Clones.- Pluripotency: Capacity for In Vitro Differentiation of Undifferentiated Embryonic Stem Cells.- Staining Embryonic Stem Cells Using Monoclonal Antibodies to Stage-Specific Embryonic Antigens.- Analysis of the Nucleolar Compartment of the Nucleus as an Indicator of Nuclear Reprogramming After Nuclear Transfer.- Methylation-Sensitive Polymerase Chain Reaction.- Analysis of DNA Methylation Profiles in Preimplantation Embryos Using Bisulfite Mutagenesis.- Chromatin Immunoprecipitation Assay for Mammalian Tissues.- Histone Modifications and Transcription Factor Binding on Chromatin: ChIP–PCR Assays.- In Vivo Genomic Footprinting Using LM–PCR Methods.- Analyzing Histone Modification Using Crosslinked Chromatin Treated With Micrococcal Nuclease.