Human Papillomaviruses

Identification of New Papillomavirus Types.- Identification of HPV Variants.- Histochemical Analysis of Cutaneous HPV-Associated Lesions.- Histological Analysis of Cervical Intraepithelial Neoplasia.- Detection of Papillomavirus Proteins and DNA in Paraffin-Embedded Tissue Sections.- Detection and Quantitation of HPV Gene Expression Using Real-Time PCR.- Analysis of p16INK4a and Integrated HPV Genomes as Progression Markers.- Use of Biomarkers in the Evaluation of CIN Grade and Progression of Early CIN.- HPV DNA Detection and Typing in Cervical Scrapes.- HPV DNA Detection and Typing in Inapparent Cutaneous Infections and Premalignant Lesions.- Establishing HPV-Containing Keratinocyte Cell Lines From Tissue Biopsies.- Using an Immortalized Cell Line to Study the HPV Life Cycle in Organotypic “Raft” Cultures.- Differentiation of HPV-Containing Cells Using Organotypic “Raft” Culture or Methylcellulose.- Propagation of Infectious, High-Risk HPV in Organotypic “Raft” Culture.- Retrovirus-Mediated Gene Transfer to Analyze HPV Gene Regulation and Protein Functions in Organotypic “Raft” Cultures.- The HPV Xenograft Severe Combined Immunodeficiency Mouse Model.- The Cottontail Rabbit Papillomavirus Model of High-Risk HPV-Induced Disease.- Studying the HPV Life Cycle in 3A Trophoblasts and Resulting Pathophysiology.- Replication and Encapsidation of Papillomaviruses in Saccharomyces cerevisiae.- Analysis of the Regulation of Viral Transcription.- Analysis of HPV Transcription by RPA.- Analysis of Regulatory Motifs Within HPV Transcripts.- Detection of HPV Transcripts by Nested RT-PCR.- Analysis of HPV DNA Replication Using Transient Transfection and Cell-Free Assays.- Detection and Quantitation of HPV DNA Replication by Southern Blotting and Real-time PCR.-Analysis of E7/Rb Associations.- Transformation Assays for HPV Oncoproteins.- Analysis of Adeno-Associated Virus and HPV Interaction.- In Vitro Assays of Substrate Degradation Induced by High-Risk HPV E6 Oncoproteins.- Measuring the Induction or Inhibition of Apoptosis by HPV Proteins.- Codon Optimization of Papillomavirus Genes.- Generation of HPV Pseudovirions Using Transfection and Their Use in Neutralization Assays.- Generation and Applications of HPV Pseudovirions Using Vaccinia Virus.