Microtubules, in vitro

University of California, Santa Barbara, USA

Section1: Isolation, Biochemistry and Characterization of Antibodies and Isotypes
1. Preparation of Microtubule Protein and Purified Tubulin from Bovine Brain by Cycles of Assembly and Disassembly and Phosphocellulose Chromatography
Herbert P. Miller and Leslie Wilson
2. Measurement Of Ligand Binding To Tubulin By Sulfhydryl Reactivity
Adrian Begaye and Dan L Sackett
3. Isolating Tubulin From Nonneural Sources
Dan L. Sackett, Karl A. Werbovetz and Naomi S. Morrissette
4. Characterization of Anti-b -Tubulin Antibodies
Anthony J. Spano* and Anthony Frankfurter
5. Expression profiling of tubulin isotypes and microtubule interacting proteins using real-time polymerase chain reaction
Sharon Lobert, Laree Hiser and John J. Correia
6. Nondenaturing Electrophoresis As A Tool To Investigate Tubulin Complexes
Mónica López-Fanarraga, Gerardo Carranza, Raquel Castaño, Sofia Nolasco, J. Avila and J.C. Zabala
7 .Mass spectrometry analysis of c-terminal posttranslational Modifications of tubulins
Redeker, Virginie
8. Methods in Tubulin Proteomics
Leah M. Miller, Hui Xiao, Berta Burd, Susan Band Horwitz, Ruth Hogue Angeletti, Pascal Verdier-Pinard

Section 2: Microtubule Structure and Dynamics
9. Cryo-EM Studies of Microtubule Structural Intermediates and Kinetochore-Microtubule Interactions
Eva Nogales, Vincent H. Ramey and Hong-Wei Wang
10. High resolution imaging of microtubules and cytoskeleton structures by Atomic Force Microscopy
Loic Hamon, Patrick A. Curmi, David Pastré
11. Using computational modeling to understand microtubule dynamics: a primer for cell biologists
Holly V. Goodson and Ivan V. Gregoretti
12. Analysis of Dynamic Instability of Steady State Microtubules In Vitro by Video-Enhanced Differential Interference Contrast Microscopy with an Appendix by Emin Oroudjev
Mythili Yenjerla, Manu Lopus and Leslie Wilson
13. Nanometer-resolution microtubule polymerization assays using optical tweezers and microfabricated barriers
Blake D. Charlebois, Henry T. Schek III, Alan J. Hunt
14. Microtubule Dynamics Reconstituted in-vitro and Imaged y Single-Molecule Fluorescence Microscopy
Christopher Gell, Volker Bormuth, Gary J. Brouhard, Daniel N. Cohen, Stefan Diez, Claire T. Friel, Jonne Helenius, Bert Nitzsche, Heike Petzold, Jan Ribbe, Erik Schäffer, Jeffrey H. Stear, Anastasiya Trushko, Vladimir Varga, Per O. Widlund, Marija Zanic, and Jonathon Howard
15. Studying kinesin motors by optical 3D-nanometry in gliding motility assays
Bert Nitzsche, Volker Bormuth, Corina Bräuer, Jonathon Howard, Leonid Ionov, Jacob Kerssemakers, Till Korten, Cecile Leduc, Felix Ruhnow, Stefan Diez

Section 3: Drugs
16. Analysis of tubulin oligomers by Analytical Ultracentrifugation.
J.J. Correia
17. Determination of Drug Binding to Microtubules in vitro
Jennifer A. Smith and Mary Ann Jordan
18. Fluorescence Spectroscopic Methods to Analyze Drug-Tubulin Interactions
Bhabatarak Bhattacharyya, Sonia Kapoor and Dulal Panda
19. A Tubulin polymerization micro-assay used to compare ligand efficacy
Ashley Davis, Sarah Martinez, Daniel Nelson and Kim Middleton.
20. Fluorescent Taxoid Probes for Microtubule Research
Isabel Barasoain, J. Fernando Díaz, and José M. Andreu
21. The binding of vinca domain agents to tubulin: structural and biochemical studies
Anthony Cormie, Marcel Knossow, Chunguang Wang and Benoît Gigant

Section 4: Interactions with Motors and MAPs
22. Probing Interactions of Tubulin with Small Molecules, Peptides and Protein Fragments by Solution Nuclear Magnetic Resonance
Marie-Jeanne Clément, Philippe Savarin, Elisabeth Adjadj, André Sobel, Flavio Toma, Patrick A. Curmi
23. Microtubule and MAPs: thermodynamics of complex formation by AUC, ITC, fluorescence and NMR
François Devred, Pascale Barbier, Daniel Lafitte, Isabelle Landrieu, Guy Lippens and Vincent Peyrot
24. Quantitative Analysis of MAP Mediated Regulation of Microtubule Dynamic Instability In Vitro – Focus on Tau
Erkan Kiris, Donovan Ventimiglia and Stuart C. Feinstein
25. Structure and dynamics of the kinesin-microtubule interaction revealed by fluorescence polarization microscopy (FPM)
Hernando Sosa, Ana B. Asenjo and Erwin J.G. Peterman
26. Multiple color single molecule TIRF imaging and tracking of MAPs and motors
Jennifer L. Ross and Ram Dixit
27. Fluorescence microscopy assays on chemically functionalized surfaces for quantitative imaging of microtubule, motor and +TIP dynamics
Peter Bieling, Ivo A. Telley, Christian Hentrich, Jacob Piehler and Thomas Surrey

Section 5: Functional Extracts and Force Measurements
28. Quantitative Characterization of filament dynamics by single-molecule lifetime measurements.
Leonid A. Mirny and Daniel J. Needleman
29. Extracting the mechanical properties of microtubules from thermal fluctuation measurements on an attached tracer particle. =
Katja M. Taute, Francesco Pampaloni, Ernst-Ludwig Florin
30. In vitro assays to study force generation at dynamic microtubule ends
Liedewij Laan and Marileen Dogterom
31. Reconstitution and functional analysis of kinetochore subcomplexes
Daniel R. Gestaut, Jeremy Cooper, Charles L. Asbury, Trisha N. Davis and Linda Wordeman
32. In vitro assays to study the tracking of shortening microtubule ends and to measure associated forces
Ekaterina L. Grishchuk and Fazly I. Ataullakhanov