Extracellular Matrix Protocols

Molecular Biology.- Retroviral delivery of ECM genes.- Tissue-Specific KO of ECM Proteins.- Recombinant Collagen Trimers from Insect Cells and Yeast.- Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag.- Preparation of recombinant fibronectin fragments for functional and structural studies.- Quantitative Determination of Collagen Cross-links.- Biochemical and biophysical analysis.- ECM Macromolecules: Height-mapping and Nano-mechanics Using Atomic Force Microscopy.- Atomic Force Microscopy Measurements of Intermolecular Binding Forces.- Mass-Mapping of ECM Macromolecules by Scanning Transmission Electron Microscopy.- Chemical Microscopy of Biological Samples by Dynamic Mode Secondary Ion Mass Spectrometry (SIMS).- ECM Macromolecules: Rotary Shadowing and Transmission Electron Microscopy.- Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Substrates.- Solid Phase Assays for Studying ECM Protein–Protein Interactions.- Cell Adhesion Assays.- Cell biology assays.- ECM Degradation Assays for Analyzing Local Cell Invasion.- Fluorescence-Based Assays for In Vitro Analysis of Cell Adhesion and Migration.- Fibrin Gel Model for Assessment of Cellular Contractility.- Fluorescent Labeling Techniques for Investigation of Fibronectin Fibrillogenesis (Labeling Fibronectin Fibrillogenesis).- Stromagenesis During Tumorigenesis: Characterization of Tumor-associated Fibroblasts and Stroma-derived 3D Matrices.- Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes.- Organ models.- ECM and FGF-Dependent Assay of Embryonic SMG Epithelial Morphogenesis: Investigating Growth Factor/Matrix Regulation of Gene Expression During Submandibular Gland Development.- Analyzing how Cell AdhesionControls Mammary Gland Function by Transplantation of Embryonic Mammary Tissue from Knockout Mice.- Tissue Engineering.- Characterizing ECM Production by Cells Encapsulated in Hydrogels.- Tissue Engineering and Cell-Populated Collagen Matrices.

From the reviews of the second edition:

“Significant advances in the field of extracellular matrix during the past decade and current extensive investigations in this field have necessitated the second edition of this book on extracellular matrix protocols. … well illustrated with of figures, photographs, and graphs in color and in black-and-white. … students interested in cell biology will find this book quite useful. … this second edition is surely welcome in the research community. Both expert and nonexpert researchers in the field of extracellular matrix will find this book extremely useful.” (Omer Iqbal, Doody’s Review Service, July, 2009)