Myeloid Leukemia -

Myeloid Leukemia

Methods and Protocols
Buch | Hardcover
307 Seiten
2005
Humana Press Inc. (Verlag)
978-1-58829-485-2 (ISBN)
160,49 inkl. MwSt
Brings together a collection of reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with the broad spectrum of myeloid malignancies. In addition to gene rearrangements, this work also covers other prognostically relevant molecular lesions such as FLT3 mutations and WT-1 overexpression.
A panel of internationally recognized research scientists and clinical investigators brings together a diverse collection of readily reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with the broad spectrum of myeloid malignancies. Highlights include techniques for the detection of BCR-ABL mutations and resistance to imatinib mesylate, detection of the FIP1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia, classification of AML by DNA-oligonucleotide microarrays, and detection of the V617F JAK2 mutation in myeloproliferative disorders. In addition to gene rearrangments, other prognostically relevant molecular lesions such as FLT3 mutations and WT-1 overexpression are covered.

Isolation of RNA and DNA From Leukocytes and cDNA Synthesis.- Cytogenetic and FISH Techniques in Myeloid Malignancies.- Overview of Real-Time RT-PCR Strategies for Quantification of Gene Rearrangements in the Myeloid Malignancies.- Diagnosis and Monitoring of Chronic Myeloid Leukemia by Qualitative and Quantitative RT-PCR.- Detection of BCR-ABL Mutations and Resistance to Imatinib Mesylate.- Deletion of the Derivative Chromosome 9 in Chronic Myeloid Leukemia.- Diagnosis and Monitoring of PML-RARA-Positive Acute Promyelocytic Leukemia by Qualitative RT-PCR.- Diagnosis and Monitoring of PML-RAR?-Positive Acute Promyelocytic Leukemia by Quantitative RT-PCR.- Diagnosis and Monitoring of AML1-MTG8 (ETO)-Positive Acute Myeloid Leukemia by Qualitative and Real-Time Quantitative RT-PCR.- Diagnosis and Monitoring of CBFB-MYH11-Positive Acute Myeloid Leukemia by Qualitative and Quantitative RT-PCR.- Detection of the FIP1L1-PDGFRA Fusion in Idiopathic Hypereosinophilic Syndrome and Chronic Eosinophilic Leukemia.- FLT3 Mutations in Acute Myeloid Leukemia.- WT-1 Overexpression in Acute Myeloid Leukemia and Myelodysplastic Syndromes.- Classification of AML by DNA-Oligonucleotide Microarrays.- Classification of AML Using a Monoclonal Antibody Microarray.- Methods for the Detection of the JAK2 V617F Mutation in Human Myeloproliferative Disorders.- Overexpression of PRV-1 Gene in Polycythemia Rubra Vera and Essential Thrombocythemia.- Chimerism Analysis Following Nonmyeloablative Stem Cell Transplantation.

Reihe/Serie Methods in Molecular Medicine ; 125
Zusatzinfo 320 p. 69 illus.
Verlagsort Totowa, NJ
Sprache englisch
Maße 155 x 235 mm
Themenwelt Medizinische Fachgebiete Innere Medizin Hämatologie
Medizin / Pharmazie Medizinische Fachgebiete Onkologie
ISBN-10 1-58829-485-4 / 1588294854
ISBN-13 978-1-58829-485-2 / 9781588294852
Zustand Neuware
Haben Sie eine Frage zum Produkt?
Mehr entdecken
aus dem Bereich
Hämatologie und Internistische Onkologie

von Dietmar P. Berger; Monika Engelhardt; Justus Duyster

Buch | Softcover (2023)
ecomed-Storck GmbH (Verlag)
129,99