HIV Protocols

Infectious Virus Tracking by Fluorescent Live Cell Imaging in Primary Cells.- Imaging HIV-1 Nuclear Import, Uncoating, and Proviral Transcription.- RNA-FISH for HIV Transcription/Localization Analysis.- Single-Cell and Single-Molecule RNA-FISH Combined with Immunofluorescence and High-Speed and High-Resolution Scanning Analysis to Visualize Reactivation of Latent HIV-1.- Visualizing HIV-1 Assembly at the T Cell Plasma Membrane using Single Molecule Localization: TIRF Microscopy.- Single-Virion Analysis: A Method to Visualize HIV-1 Particle Content Using Fluorescence Microscopy.- Correlative Imaging to Detect Rare HIV Reservoirs and Associated Damage in Tissues.- Characterization of Nuclear HIV-Induced Membraneless Organelles through Fluorescence Microscopy.- Detection of CPSF6 in Biomolecular Condensates as a Reporter of Nuclear Import.- Monitoring HIV-1 Nuclear Import Kinetics Using a Chemically Induced Nuclear Pore Blockade Assay.- Biochemical Detection of Capsid in the Nucleus During HIV-1 Infection.- Chromatin Immunoprecipitation of Retroviral Genomes with Antibodies Recognizing Modified Histones and Specific Viral Proteins.- Measurement of HIV Rev-Rev Response Element Functional Activity.- Method for the Enrichment of N6-Methyladenosine-Modified Cellular and HIV-1 RNA.- Direct Analysis of HIV mRNA m6A Methylation by Nanopore Sequencing.- Identifying Individual Pseudouridine (Ψ) Sites Across Transcripts from HIV-1 Infected Cells.- Proximity Ligation Assay to Detect the Proximity between Host Proteins and Viral Proteins of HIV-1.- Modeling HIV-1 Infection in CNS via Infected Monocytes using Immunocompetent Brain Organoids.- An In Vitro Model of Blood Brain Barrier for Studies on HIV Neuroinflammation and CNS Antibody Penetration.- Generation of Anti-HIV CAR-T Cells for Preclinical Research.- Highly Sensitive Analysis of Cervical Mucosal HIV-1 Infection using Reporter Viruses Expressing Secreted Nanoluciferase.- Multiparameter Flow Cytometry Monitoring of T Cell Responses.- An Ultrasensitive p24 Assay to Measure HIV-1 in Diverse Biological Matrixes.