The Tumor Microenvironment

Imaging Mass Cytometry in Immuno-oncology.- Analyzing the Tumor Immune Microenvironment by Flow Cytometry.- Blood sample processing and banking for functional and molecular analyses.- Quantitatively Assessing the Respiratory Burst in Innate Immune Cells.- Visualizing NETosis using a Novel Neutrophil Extracellular Trap-Specific Marker.- In vitro methods to evaluate macrophage polarization and function in cancer.- In vivo analysis of tumor associated macrophages in the tumor microenvironment.- Assessing the immune regulatory role of metabolites in vitro.- Mouse Models of Obesity To Study The Tumor Immune Microenvironment.- Mouse Models of Obesity To Study The Tumor Immune Microenvironment.- Assessing the efficacy of immune checkpoint inhibitors in pre-clinical tumor models.- Fibroblast isolation from mammary gland tissue and syngeneic murine breast cancer models.- Multiscale label-free imaging of fibrillar collagen in the tumor microenvironment.- In situ measurement of intra-tumoral tissue rigidity.- Extracellular matrix glycation and crosslinking in mammary tumor progression.- Proteomic assessment of the murine mammary gland extracellular matrix.- Spheroids as a 3D Model of the Hypoxic Tumor Microenvironment.- Tumor pH imaging using Chemical Exchange Saturation Transfer (CEST)-MRI.- Profiling the epigenetic landscape of the tumor microenvironment using chromatin immunoprecipitation-sequencing.- Analysis of Spatial Molecular Data.- Laser-assisted single-cell labeling and capture.- 3D hydrogel cultures for high-throughput drug discovery.- Intravital microscopy of the metastatic pulmonary environment.- A CRISPR platform for targeted in vivo screens.