Experimental Neurotoxicology Methods

Amino-Cupric-Silver (A-Cu-Ag) Staining to Detect Neuronal Degeneration in the Mouse Brain: The de Olmos Technique.- Assessment of Auditory Hair Cell Loss by Cytocochleograms.- Evaluation of Cellular and Molecular Pathology in the Rodent Vestibular Sensory Epithelia by Immunofluorescent Staining and Confocal Microscopy.- Morphometric Analysis of Axons and Dendrites as a Tool for Assessing Neurotoxicity.- DPOAEs for the Assessment of Noise- or Toxicant-Induced Cochlear Damage in the Rat.- Use of Visual Evoked Potentials to Assess Deficits in Contrast Sensitivity in Rats following Neurotoxicant Exposures.- Electrophysiological Assessments in Peripheral Nerves and Spinal Cord in Rodent Models of Chemotherapy-Induced Painful Peripheral Neuropathy.- The Functional Observation Battery: Utility in Safety Assessment of New Molecular Entities.- Behavioral Assessment of Vestibular Dysfunction in Rats.- Assessment of Olfactory Toxicity in Dodents.- Assessment of Neurotoxicant-Induced Changes in Behavior: Issues Related to Interpretation of Outcomes and Experimental Design.- Assessment of Neurofilament Light Protein as a Serum Biomarker in Rodent Models of Toxic-Induced Peripheral Neuropathy.- Assessing Neurotoxicant-Induced Inflammation in the Central Nervous System: Cytokine mRNA with Immunostaining of Microglia Morphology.- Mitochondrial Stress Assay and Glycolytic Rate Assay in Microglia using Agilent Seahorse Extracellular Flux Analyzers.- Assessment of Larval Zebrafish Locomotor Activity for Developmental Neurotoxicity Screening.- A Behavioral Test Battery to Assess Larval and Adult Zebrafish after Developmental Neurotoxic Exposure.- Evaluation of Neurotoxic Effects in Zebrafish Embryos by Automatic Measurement of Early Motor Behaviors.- Application of Fluorescence Microscopy and Behavioral Assays to Demonstrating Neuronal Connectomes and Neurotransmitter Systems in C. elegans.- Effects of Neurotoxic or Pro-Regenerative Agents on Motor and Sensory Neurite Outgrowth in Spinal Cord Organotypic Slices and DRG Explants in Culture.- Integrative In Vitro/Ex Vivo Assessment of Dopaminergic Neurotoxicity in Rodents using Striatal Synaptosomes and Membrane Preparations.- Quantification of Oligodendrocytes and Myelin in Human iPSC-Derived 3D Brain Cell Cultures (Brainspheres).- Measurement of Electrical Activity of Differentiated Human iPSC-Derived Neurospheres Recorded by Microelectrode Arrays (MEA).