Trypanosomatids

Field Isolation and Cultivation of Trypanosomatids from Insects.- Culturing and Transfection of Pleomorphic Trypanosoma brucei.- In Vitro Culture for Differentiation Simulation of Leishmania spp..- Tsetse Fly Transmission Studies of African Trypanosomes.- Infecting Triatomines with Trypanosomes.- High-Throughput Sequencing for Trypanosome Transcriptome Characterization.- Polysome Profiling and Metabolic Labelling Methods to Measure Translation in Trypanosoma brucei.- Immunoprecipitation for the Analysis of Macromolecular Complexes in Trypanosoma cruzi.- Analysis of the In Vivo Translation Process in Trypanosoma cruzi using Ribosome Profiling.- Proteome-Wide Quantitative Phosphoproteomic Analysis of Trypanosoma brucei Insect and Mammalian Lifecycle Stages.- Genome-Wide Proteomics and Phosphoproteomics Analysis of Trypanosoma cruzi during Differentiation.- Genome-Wide Proteomics and Phosphoproteomics Analysis of Leishmania spp. during Differentiation.- CRISPR/Cas9 Technology Applied to the Study of Proteins Involved in Calcium Signaling in Trypanosoma cruzi.- Application of CRISPR/Cas9 Mediated Genome Editing in Leishmania.- Next Generation Analysis of Trypanosomatid Genome Stability and Instability.- Transcription Factor Analysis in Trypanosomatids.- Identifying Trypanosome Protein-RNA Interactions using RIP-Seq.- The Tethering Assay: A Simple Method for the Characterization of mRNA-Fate Regulators.- RNA-Binding Proteins and their Targets in Trypanosoma brucei: Single Nucleotide Resolution using iCLIP and iCLAP.- In Vivo Tethering System to Isolate RNA-Binding Proteins Regulating mRNA Decay in Leishmania.- Forward Genetics in African Trypanosomes.- Analysis of Base Excision and Single Strand Break Repair Activities in Trypanosomatid Extracts.- Light Microscopy in Trypanosomes: Use of Fluorescent Proteins and Tags.- ImageJ for Partially and Fully Automated Analysis of Trypanosome Micrographs.- Motility Analysis of Trypanosomatids.- Electron Microscopy Techniques Applied to Symbiont-Harboring Trypanosomatids: The Association of the Bacterium with Host Organelles.- AiryScan Super-Resolution Microscopy to Study Trypanosomatid Cell Biology.- All You Ever Wanted to Know About APOL1 and TLFs and Didn’t Dare Ask.- Isolation of Leishmania Promastigote Flagella.- Gel Based Methods for the Investigation of Signal Transduction Pathways in Trypanosoma brucei.- Methods to Investigate Signal Transduction Pathways in Trypanosoma cruzi: Cyclic Nucleotide Phosphodiesterases Assay Protocols.- Methods for the Investigation of Trypanosoma cruzi Amastigote Proliferation in Mammalian Host Cells.- Isolation of Extracellular Vesicles from Leishmania spp..- 2D Gel Electrophoresis Analysis of Leishmania Proteomes.- Analysisof the Physiological and Metabolic State of Leishmania using Heavy Water Labelling.- A Scalable Purification Method for Mitochondria from Trypanosoma brucei.- Isolation of Glycosomes from Trypanosoma cruzi.- Sorting the Muck from the Brass: Analysis of Protein Complexes and Cell Lysates.- Measurement of Energy States of the Trypanosomatid Mitochondrion.- Isolation and Characterization of Acidocalcisomes from Trypanosomatids.- Metabolic Control Analysis for Drug Target Prioritization in Trypanosomatids.- Establishment of a Standardized Vaccine Protocol for the Analysis of Protective Immune Responses during Experimental Trypanosome Infections in Mice.- Isolation of Trypanosoma brucei brucei Infection-Derived Splenic Marginal Zone B Cells Based on CD1dHigh/B220High Surface Expression in a Two-Step MACS-FACS Approach.- Cellular Markers for the Identification of Chemo-Resistant Isolates in Leishmania.- In Vitro Image-Based Assay for Trypanosoma cruzi Intracellular Forms.- In Vitro Drug Efficacy Testing against Trypanosoma brucei.- In Vitro Growth Inhibition Assays of Leishmania spp.- In Vivo Bioluminescence Imaging to Assess Compound Efficacy against Trypanosoma brucei.