Ribozymes

Conjugation as a Highly Sensitive Assay to Study Group II Intron Splicing in vivo.- Co-transcriptional Analysis of Self-cleaving Ribozymes and their Ligand Dependence.- Cloning and Detection of Genomic Retrozymes and their circRNA Intermediates.- Demonstration of a Ribozyme in Epsilon Domain of Hepatitis B Virus RNA.- In vitro Selection of Varkud Satellite Ribozyme Variants that Cleave a Modified Stem-loop Substrate.- Characterization and Optimization of a Deoxyribozyme with a Short Left Binding Arm.- Computer-aided Design of Active Pseudoknotted Hammerhead Ribozymes.- Inverse RNA Folding Workflow to Design and Test Ribozymes Including Pseudoknots.- Using an L7Ae-tethered, Hydroxyl Radical-Mediated Footprinting Strategy to Identify and Validate Kink-Turns in RNAs.- SHAPE Profiling to Probe Group II Intron Conformational Dynamics during Splicing.- Dynamics-Function Analysis in Catalytic RNA Using NMR Spin Relaxation and Conformationally Restricted Nucleotides.- Design and Evaluation of Guide RNA Transcripts with a 3’-terminal HDV Ribozyme to Enhance CRISPR-based Gene Inactivation.- Design and Evaluation of AgoshRNAs with 3’-terminal HDV Ribozymes to Enhance the Silencing Activity.- Cloning and Detection of Aptamer-ribozyme Conjugations.- Use of a Lariat Capping Ribozyme to Study Cap Function in Vivo.- Long Non-coding RNA Depletion using Self-cleaving Ribozymes.