Extracellular RNA
Humana Press Inc. (Verlag)
978-1-4939-8535-7 (ISBN)
Authoritative and cutting-edge, Extracellular RNA: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Extracellular RNAs – A New Awareness of Old Perspectives.- Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles.- Extracellular RNA Isolation from Cell Culture Supernatant.- Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture.- Isolation of Extracellular RNA from Serum/Plasma.- Isolation of Extracellular RNA from Bile.- Cushioned–Density Gradient Ultracentrifugation (C–DGUC): a Refined and High Performance Method for the Isolation, Characterization & Use of Exosomes.- Magnetic Particle-Based Immunoprecipitation of Nanoscale Extracellular Vesicles from Biofluids.- Enrichment of Extracellular Vesicle Subpopulations via Affinity Chromatography.- Detection and Analysis of Non-vesicular Extracellular RNA.- Isolation of Plasma Lipoproteins as a Source of Extracellular RNA.- Droplet Digital PCR for Quantitation of Extracellular RNA.- Preparation of Small RNA NGS Libraries from Biofluids.- Multiplexed Detection and Quantitationof Extracellular Vesicle RNA Expression using NanoString.- Milk-derived Extracellular Vesicles for Therapeutic Delivery of Small Interfering RNAs.- Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs.
Erscheinungsdatum | 19.03.2019 |
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Reihe/Serie | Methods in Molecular Biology ; 1740 |
Zusatzinfo | 30 Illustrations, color; 13 Illustrations, black and white; XI, 216 p. 43 illus., 30 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
Schlagworte | Biomarkers • density gradient ultracentrifugation • exRNA • Nanosight • Nanostring • RNA isolation |
ISBN-10 | 1-4939-8535-3 / 1493985353 |
ISBN-13 | 978-1-4939-8535-7 / 9781493985357 |
Zustand | Neuware |
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