Bacterial Polysaccharides

1. Customisable cloning of whole polysaccharide gene clusters by yeast homologous recombination.- 2. RecET Direct Cloning of Polysaccharide Gene Cluster from Gram-negative Bacteria.- 3. Purification of Capsular Polysaccharide Complex from Gram Negative Bacteria.- 4. Simple protocol to purify cell wall polysaccharide from Gram+ bacteria and assess its structural integrity.- 5. Extraction and purification of wall bound polymers of Gram-positive bacteria.- 6. Purification and Analysis of Mycobacterial Phosphatidylinositol Mannosides, Lipomannan and Lipoarabinomannan.- 7. Conjugation of synthetic oligosaccharides to proteins by squaric acid chemistry.- 8. Direct conjugation of bacterial polysaccharides to proteins by squaric acid chemistry.- 9. Chemical Modification of the N-Acetyl Moieties of Hyaluronic Acid from Streptococcus equi for studies in cytokine production.- 10. O-Acetylation of Bacterial Cell Wall Polysaccharides.- 11. Designing glycosyltransferase expression constructs for improved purification, protein yield and crystallization.- 12. Utilization of fluorescently-tagged synthetic acceptor molecules for in vitro characterization of a dual-domain glycosyltransferase enzyme, KpsC, from Escherichia coli.- 13. Synthesis of Phenoxyundecyl Diphosphate Disaccharides for Studies of the Biosynthesis of O-Antigenic Polysaccharides in Enteric Bacteria.- 14. Characterization of arabinosyl transfer reactions in the biosynthesis of mycobacterial cell envelope (lipo)polysaccharides.- 15. Enzymatic synthesis of the repeating unit oligosaccharides of Escherichia coli O104.- 16. Sulfhydryl labelling as a tool to investigate the topology of membrane proteins involved in lipopolysaccharide biosynthesis.- 17. Assay Methods for the Glycosyltransferases Involved in Synthesis of Bacterial Polysaccharides.- 18. Glycosyltransferase Activity Assays Using Colorimetric Methods.-  19. In vitro characterization of a multidomain glycosyltransferase using fluorescently-tagged synthetic acceptors.- 20. Glycosyltransferase-coupled assays for 4-epimerase WbpP from Pseudomonas aeruginosa.- 21. Assaying fucosidase activity.- 22. In vitro synthesis of bioactive glycovariants of enterocin 96, an antimicrobial peptide from Enterococcus faecalis.- 23. High-Throughput Screening for Inhibitors of Wall Teichoic Acid Biosynthesis in Staphylococcus aureus.