Immune Checkpoint Blockade

Double Immunohistochemistry and Digital Image Analysis.- Multispectral Fluorescence Imaging Allows for Distinctive Topographic Assessment and Sub-Classification of Tumor Infiltrating and Surrounding Immune Cells.- Systems Level Immune Monitoring by Mass Cytometry.- Immune Monitoring of Cancer Patients by Multi-Color Flow Cytometry.- High-Throughput, Fast and Sensitive Immunopeptidomics Sample Processing for Mass Spectrometry.- Identification of Immune Modulatory miRNAs by miRNA Enrichment via RNA Affinity Purification.- Expansion of Tumor-Infiltrating Lymphocytes from Melanoma Tumors.- Establishment of Bi-Dimensional (2D) and Three-Dimensional (3D) Melanoma Primary Cultures as a Tool for In Vitro Drug Resistance Studies.- Assessment of Anti-Tumor T Cell Responses by Flow Cytometry after Co-Culture of Tumor Cells with Autologous Tumor-Infiltrating Lymphocytes.- Expansion and Determination of Antigen-Reactive T Cells by Flow Cytometry.- Assessing Canonical and Adaptive Natural Killer Cell Function in Suppression Assays In Vitro.- Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Chromium Release Assay.- Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Flow Cytometry.- Evaluation of Breast Cancer and Melanoma Metastasis in Syngeneic Mouse Models.- Establishment of Melanoma Tumor Xenograft Using Single Cell Line Suspension and Co-Injection of Patient-Derived T Cells in Immune Deficient NSG Mice.- Analysis of Tissue Resident Immune Cells from Mouse Skin and Lungs by Flow Cytometry.