High-Throughput RNAi Screening
Humana Press Inc. (Verlag)
978-1-4939-8168-7 (ISBN)
Cutting-edge and thorough, High-Throughput RNAi Screening: Methods and Protocols provides a comprehensive source of protocols and other necessary information to make robust and successful assays possible for all who wish to apply HT-RNAi in their research.
Establishing an Infrastructure for High-Throughput Short-Interfering RNA Screening.- Optimization of Transfection Conditions for siRNA Screening.- High Throughput siRNA Screening Using Reverse Transfection.- Genome-Wide siRNA Screening Using Forward Transfection: Identification of Modulators of Membrane Trafficking in Mammalian Cells.- Pooled shRNA Screening in Mammalian Cells as a Functional Genomic Discovery Platform.- A Protocol for a High-Throughput Multiplex Cell Viability Assay.- RNAi Screening of Leukemia Cells Using Electroporation.- Self-Assembled Cell Microarray (SAMcell) for High-Throughput RNAi Screening.- In Vitro Pooled shRNA Screening to Identify Determinants of Radiosensitivity.- Three-Dimensional Spheroid Cell Culture Model for Target Identification Utilizing High-Throughput RNAi Screens.- In Vitro High Throughput RNAi Screening to Accelerate the Process of Target Identification and Drug Development.- High-Throughput, Liquid-Based Genome-Wide RNAi Screening in C. elegans.- Design and Methods of Large-Scale RNA Interference Screens in Drosophila.- Genome-Wide RNAi Screening in C. elegans for Aging and Innate Immunity.- RNAi-Assisted Genome Evolution (RAGE) in Saccharomyces cerevisiae.- RNAi Screening in Spodoptera frugiperda.- Morphology and Gene Expression Screening with Morpholinos in Zebrafish Embryos.- Live Cell Microscopy-Based RNAi Screening in the Moss Physcomitrella patens.- Data Analysis For High Throughput RNAi Screening.
Erscheinungsdatum | 25.08.2018 |
---|---|
Reihe/Serie | Methods in Molecular Biology ; 1470 |
Zusatzinfo | 43 Illustrations, color; 9 Illustrations, black and white; XI, 262 p. 52 illus., 43 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
Schlagworte | assay development • C. elegans • Drosophila • drug discovery • drug sensitizer identification • genomics • HT-RNAi • library screening • Mammalian Cells • short hairpin RNA (shRNA) • short interfering RNA (siRNA) |
ISBN-10 | 1-4939-8168-4 / 1493981684 |
ISBN-13 | 978-1-4939-8168-7 / 9781493981687 |
Zustand | Neuware |
Haben Sie eine Frage zum Produkt? |
aus dem Bereich