Cystic Fibrosis

Introduction to Part I: Overview of Approaches to Study Cystic Fibrosis Pathophysiology.- Imaging CFTR Protein Localization in Cultured Cells and Tissues.- CFTR Regulation of Epithelial Sodium Channel.- Methods for Evaluating Inflammation in Cystic Fibrosis.- Methods for ASL Measurements and Mucus Transport Rates in Cell Cultures.- Measurement of Fluid Secretion from Intact Airway Submucosal Glands.- Measurements of Intracellular Calcium Signals in Polarized Primary Cultures of Normal and Cystic Fibrosis Human Airway Epithelia.- Identification and Quantification of Mucin Expression.- Methods to Classify Bacterial Pathogens in Cystic Fibrosis.- Approaches to Study Differentiation and Repair of Human Airway Epithelial Cells.- Introduction to Part II: Omics in the Biology of Cystic Fibrosis.- Microarray mRNA Expression Profiling to Study Cystic Fibrosis.- Quantitative Differential Proteomics of Cystic Fibrosis Cell Models by SILAC (Stable Isotope Labelling in Cell Culture).- Application of Mass Spectrometry to Study Proteomics and Interactomics in Cystic Fibrosis.- Functional Genomics Assays to Study CFTR Traffic and ENaC Function.- New Lipidomic Approaches in Cystic Fibrosis.- Introduction to Part III: Resources for CFTR Research.- Primary Epithelial Cell Models for Cystic Fibrosis Research.- Comparative Biology of Cystic Fibrosis Animal Models.- CFTR Folding Consortium: Methods Available for Studies of CFTR Folding and Correction.- Evaluation of the Disease Liability of CFTR Variants.