Immunogenetics

The Major Histocompatibility Complex - A Paradigm for Studies of the Human Genome.- HLA Typing by SSO and SSP Methods.- Methods for Diagnostic HLA Typing in Disease Association and Drug Hypersensitivity.- HLA Typing Using Bead Based Methods.- HLA Typing by Direct DNA Sequencing.- Data Analysis of HLA Sequencing using Assign-SBT v3.6+ from Conexio.- Simple Methods for the Detection of HLA-G Variants in Coding and Non-Coding Regions.- Molecular Typing of HLA-E.- Molecular Analysis of Complement Component C4 Gene Copy Number.- Genotyping of Single Nucleotide Polymorphisms (SNPs) by 5’ Nuclease Allelic Discrimination.- High Resolution MICA Genotyping by Sequence-Based Typing (SBT).- Standard Methods for the Management of Immunogenetic Data.- Analytical Methods for Immunogenetic Population Data.- Analytical Methods for Disease Association Studies with Immunogenetic Data.- Impact of HLA Matching and HLA Antibodies in Organ Transplantation: a Collaborative Transplant Study View.- Screening for Antibodies Against MICA by Luminex Flow Cytometry.- HLA Antibody Detection and Characterization by Solid Phase Immunoassays - Methods and Pitfalls.- Detection and Characterisation of Alloreactive T Cells.- Detection of Allo-HLA Crossreactivity by Viral Specific Memory T-Cell Clones using Single HLA Transfected K562 Cells.- Separation and Cryopreservation of Lymphocytes from Spleen and Lymph Node.- Crossmatching by Complement-Dependent Lymphocytotoxicity (CDC).- The Lymphocyte Crossmatch by Flow Cytometry for Kidney Transplantation.- Overview of the Killer Cell Immunoglobulin-Like Receptor (KIR) System.- KIR Typing by Non Sequencing Methods: Polymerase-Chain Reaction with Sequence-Specific Primers (PCR-SSP).- Killer Cell Immunoglobulin-Like Receptors (KIR) Typing by DNA Sequencing.- An Overview of Methods Required to Evaluate Donor NK Cell Alloreactivity for Haploidentical Haemopoietic Stem Cell Transplantation.- The Detection of NK Cell Alloreactivity by Flow CytometricCD107a Assay.- Clinical Production and Therapeutic Applications of Alloreactive Natural Killer Cells.- Minor Histocompatibility Antigen Typing by DNA Sequencing for Clinical Practice in Hematopoietic Stem-Cell Transplantation.- Donor Registries and Search Strategies.- Cytokine Gene Polymorphisms: Methods of Detection and Biological Significance.- IMGT® Tools for the Nucleotide Analysis of Immunoglobulin (IG) and T Cell Receptor (TR) V-(D)-J Repertoires, Polymorphisms, and IG Mutations: IMGT/V-QUEST and IMGT/HighV-QUEST for NGS.- IMGT/DomainGapAlign: the IMGT® Tool for the Analysis of IG, TR, MH, IgSF and MhSF Domain Amino Acid Polymorphism.- Human Gm, Km and Am Allotypes and their Molecular Characterization: a Remarkable Demonstration of Polymorphism.