Spliceosomal Pre-mRNA Splicing

The Pre-mRNA Splicing Reaction.- Diversity and Evolution of Spliceosomal Systems.- Mechanisms of Spliceosomal Assembly.- Alternative Pre-mRNA Splicing.- Regulation of Alternative Pre-mRNA Splicing.- Introduction to Co-Transcriptional RNA Splicing.- Chromatin and Splicing.- Preparation of Splicing Competent Nuclear Extracts.- Preparation of Yeast Whole Cell Splicing Extract.- Efficient Splinted Ligation of Synthetic RNA Using RNA Ligase.- In Vitro Assay of Pre-mRNA Splicing in Mammalian Nuclear Extract.- Kinetic Analysis of In Vitro Pre-mRNA Splicing in HeLa Nuclear Extract.- In Vitro Systems for Coupling RNAP II Transcription to Splicing and Polyadenylation.- Isolation and Accumulation of Spliceosomal Assembly Intermediates.- Complementation of U4 snRNA in S. cerevisiae Splicing Extracts for Biochemical Studies of snRNP Assembly and Function.- Expression and Purification of Splicing Proteins from Mammalian Cells.- Single Molecule Approaches for Studying Spliceosome Assembly and Catalysis.- Cell-Based Splicing of Minigenes.- Quantifying the Ratio of Spliceosome Components Assembled on Pre-mRNA.- Antisense Methods to Modulate Pre-mRNA Splicing.- Using Yeast Genetics to Study Splicing Mechanisms.- Medium Throughput Analysis of Alternative Splicing by Fluorescently Labeled RT-PCR.- Chromatin Immunoprecipitation Approaches to Determine Co-Transcriptional Nature of Splicing.- Computational Approaches to Mine Publicly Available Databases.- Approaches to Link RNA Secondary Structures with Splicing Regulation.- Methods to Study Splicing from High-Throughput RNA Sequencing Data.- Global Protein-RNA Interaction Mapping at Single Nucleotide Resolution by iCLIP-Seq.- Predicting Alternative Splicing.