Advances and Clinical Practice in Pyrosequencing

Preparation of single-stranded DNA for pyrosequencing by LATE-PCR.- A simplified protocol for preparing pyrosequencing templates based on LATE-PCR using whole blood as starting material directly.- Improvement of LATE-PCR to prepare pyrosequencing template.- Pyrosequencing-templates generated by nicking PCR products with nicking endonuclease.- Pyrosequencing-templates generated by asymmetric Nucleic Acid Sequence-Based Amplification (asymmetric-NASBA).- Gel immobilization of acrylamide-modified single-stranded DNA template for pyrosequencing.- Multiplex PCR based on a universal biotinylated primer to generate templates for pyrosequencing.- A novel pyrosequencing principle based on AMP-PPDK reaction for improving the detection limit.- Pyrosequencing chemistry coupled with modified primer extension reactions for quantitative detection of allele frequency in a pooled sample.- A gel-free SNP genotyping method based on pyrosequencing chemistry coupled with modified primer extension reactionsdirectly from double-stranded PCR products.- Improvement of pyrosequencing to allow multiplex SNP typing in a program.- Improvement of pyrosequencing-sensitivity based on the capture of free adenosine 5'-phosphosulfate with adenosine triphosphate sulfurylase.- Using polymerase preference index to design imLATE-PCR primers for an efficient pyrosequencing.- Preparation of thermalstable biotinlyted firefly luciferase and its application in pyrosequencing.- Expression of thermostable recombinant luciola lateralis luciferase and its function on setting up of heat-stable pyrosequencing system.- Expression and purification of ATP sulfurylase from saccharomyces cerevisias in Escherichia coli and its application in pyrosequencing.- Characterization of recombinant Escherichia coli single-strand binding protein and its application in pyrosequencing.- Expression of PPDK from Microbispora Rosea Subsp.aerata in E.coli and its application in pyrosequencing.- Construction of 3-plex barcodes for differential gene-expression analysis with pyrosequencing.- Development of pyrosequencing-based multiplex bioassay by designing barcodes encoded with artificially designed sequences.- Quantitatively discriminating multiplexed LAMP products with pyrosequencing-based bio-barcodes.- Multiplex ligation-dependent sequence-tag amplification coupled with pyrosequencing for CNV analysis.- Pyrosequencer miniaturized with capillaries to deliver deoxynucleotides.- Pyrosequencer with a senor of a photo-diode array.- Pyrosequencing on acryl-modified glass chip.- Pyrosequencing on-chip based on a gel-based solid-phase amplification.- Prenatal diagnosis of trisomy 21 by quantitatively pyrosequencing heterozygotes using amniotic fluid as starting material of PCR.- Comparative gene expression analysis of breast-cancer related genes by multiplex pyrosequencing coupled with sequence-barcodes.- MicroRNA quantification by pyrosequencing with a sequence-tagged stem-loop RT primer.- Analysis of genetically modified organisms by pyrosequencing on a portable photodiode-based bioluminescence sequencer.- Genotyping of pathogenic serotypes of S. suis with pyrosequencing.- Differential gene expression analysis of breast cancer by combining sequence-tagged reverse-transcription PCR with pyrosequencing.- Detection of avian influenza A virus by pyrosequencing.- Genotyping of the alcohol dehydrogenase gene by pyrosequencing coupled with improved LATE-PCR using human whole blood as starting material.- Pyrosequencing on a single cell.