Molecular Pathology Protocols

DNA Extraction from Paraffin-Embedded Tissues.- DNA Extraction from Fresh or Frozen Tissues.- RNA Extraction from Fresh or Frozen Tissues.- Single-Strand Conformation Polymorphism Analysis of Mutations in Exons 4-8 of the TP53 Gene.- Cleavase® Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection.- Detection of Telomerase by In Situ Hybridization and by the Polymerase Chain Reaction-Based Telomerase Activity Assay.- Detection of Microsatellite Instability.- Polymerase Chain Reaction Clonality Assays Based on X-Linked Genes.- Fluorescent In Situ Hybridization.- HER-2/neu Oncogene Amplification Determined by Fluorescence In Situ Hybridization.- A Nested Reverse Transcription-Polymerase Chain Reaction Assay to Detect BCR/abl.- Detection of t(15;17)(q24;q21), inv(16)/t(16;16)(p13;q22), and t(8;21)(q22;q22) Anomalies in Acute Myeloid Leukemias.- Detection of t(14; 18)(q32;q21)-Associated BCL-2/JH Gene Fusion in Non-Hodgkin Lymphoma.- Detection of Breast Cancer Cells Using Immunomagnetic Beads and Reverse Transcriptase Polymerase Chain Reaction.- Molecular Detection of Circulating Prostate Cancer Cells.- Methods to Detect Clonal Gene Rearrangements in Lymphomas and Leukemias.- Monitoring of Bone Marrow Transplant Engraftment.- Direct Molecular Diagnosis of Multiple Endocrine Neoplasia Type 1.- Molecular Detection of Multiple Endocrine Neoplasia Type 2.- Assay for Detecting the I1307K Susceptibility Allele within the Adenomatous Polyposis ColiGene.- Detection of Human Papillomaviruses by Polymerase Chain Reaction and In Situ Hybridization.- Molecular Methods for Detecting Epstein-Barr Virus (Part I).- Molecular Methods for Detecting Epstein-Barr Virus (Part II).- Molecular Methods for Detecting Epstein-Barr Virus (Part III).- Molecular Detection ofKaposi’s Sarcoma-Associated Herpesvirus/ Human Herpesvirus-8.- Diagnostic Applications of Quantitative Polymerase Chain Reaction for Cytomegalovirus.- A Colorimetric Microtiter Plate Polymerase Chain Reaction System That Detects Herpes Simplex Virus in Cerebrospinal Fluid and Discriminates Genotypes 1 and 2.- Detection and Typing of Hepatitis C Virus.- Detection and Speciation of Mycobacteria in Formalin-Fixed, Paraffin-Embedded Tissue Sections.- Ultrasensitive Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma by the AMPLICOR and COBAS AMPLICOR HIV-1 MONITOR™ Tests.- Molecular Diagnosis of Hereditary Thrombotic Disorders.- Prenatal Genotyping of the RhD Locus to Identify Fetuses at Risk for Hemolytic Disease of the Newborn.- Molecular Diagnosis of Hereditary Hemochromatosis.- Genotyping of Apolipoprotein E.- Genotyping for Functionally Important Human CYP2D6*4 (B) Mutation Using TaqMan Probes.