Microwave Fixation of Labile Metabolites (eBook)

Front Cover 1
Microwave Fixation of Labile Metabolites 4
Copyright Page 5
Table of Contents 8
PREFACE 6
ACKNOWLEDGEMENTS 7
CONTRIBUTORS 10
Chapter 1. Study of Brain Neurochemistry Utilizing Rapid Inactivation of Brain Enzyme Activity by Heating with Microwave Irradiation 14
ABSTRACT 14
KEYWORDS 14
INTRODUCTION 15
METHODS 15
CONCLUSIONS 21
REFERENCES 22
Chapter 2. Studies of Different Labile Pools of Acetylcholine in Brain by Using Alternative Methods of Sacrifice by Microwave Irradiation 26
ABSTRACT 26
KEYWORDS 26
INTRODUCTION 26
MATERIALS AND METHODS 27
RESULTS AND DISCUSSION 30
ACKNOWLEDGEMENTS 36
REFERENCES 36
Chapter 3. Contribution of Loeomotor Activity to Change in Cerebellar Cyclic GMP Following Administration of Drugs 38
ABSTRACT 38
KEYWORDS 38
INTRODUCTION 38
MATERIALS AND METHODS 39
RESULTS 40
DISCUSSION 41
ACKNOWLEDGEMENTS 43
REFERENCES 43
Chapter 4. The Importance of Rapid Enzymatic Inactivation in the Determination of Catecholamines in the Mouse Brain 46
ABSTRACT 46
KEYWORDS 46
INTRODUCTION 46
EXPERIMENTAL 47
RESULTS 48
DISCUSSION 51
ACKNOWLEDGEMENTS 52
REFERENCES 52
Chapter 5. Histochemical Fluorescence of Brain Catecholamines Following Microwave Irradiation 56
ABSTRACT 56
KEYWORDS 56
INTRODUCTION 56
METHODS 57
RESULTS AND DISCUSSION 58
REFERENCES 63
Chapter 6. Comparison of Central Nervous System Neurotransmitter Levels in the Rat Brain Following Decapitation and Microwave Irradiation 66
ABSTRACT 66
KEYWORDS 66
INTRODUCTION 66
MATERIALS AND METHODS 67
RESULTS AND DISCUSSION 67
REFERENCES 75
Chapter 7. Microwave Heating and Serotonin Determinations 76
ABSTRACT 76
KEYWORDS 76
INTRODUCTION 76
EXPERIMENTAL 77
RESULTS 79
CONCLUSIONS 81
ACKNOWLEDGEMENTS 85
REFERENCES 85
Chapter 8. Post Mortem Changes of .-Aminobutyric Acid in the Murine Brain Following Microwave Irradiation 88
ABSTRACT 88
KEYWORDS 88
INTRODUCTION 88
MATERIALS AND METHODS 89
RESULTS AND DISCUSS0N 89
REFERENCES 92
Chapter 9. Catecholamines in the Hypothalamo-pituitary-thyroid System of Microwave Irradiated Rats 94
ABSTRACT 94
KEYWORDS 94
INTRODUCTION 95
MATERIALS AND METHODS 95
RESULTS AND DISCUSSION 96
REFERENCES 105
Chapter 10. Effect of Cholinergic Drugs, Stress and Locomotor Activity on Cyclic Nucleotides in Brain and Pituitary 108
ABSTRACT 108
KEYWORDS 109
INTRODUCTION 109
METHODS 111
RESULTS 112
DISCUSSION 118
ACKNOWLEDGEMENTS 123
REFERENCES 123
Chapter 11. Elevation of Rat Brain Histamine Content by Microwave Irradiation 130
ABSTRACT 130
KEYWORDS 130
INTRODUCTION 130
MATERIALS AND METHODS 131
RESULTS 132
DISCUSSION 135
REFERENCE 139
Chapter 12. Application of Microwave Irradiation to the Analysis of Cyclic Nucleotides in Mouse Brain During Convulsions Induced by N,N' dibenzoylguanidine 142
ABSTRACT 142
KEYWORDS 142
INTRODUCTION 142
MATERIALS AND METHODS 143
RESULTS 143
DISCUSSION 146
CONCLUSIONS 148
REFERENCES 149
Chapter 13. Microwave Irradiation Eliminates Circadian Rhythm in Whole Brain Serotonin but Facilitates Quantitation of Bound and Free Serotonin Content 150
ABSTRACT 150
KEYWORDS 150
INTRODUCTION 151
MATERIALS AND METHODS 151
RESULTS 152
DISCUSSION 154
REFERENCES 156
Chapter 14. Catecholamine Turnover in the Central Nervous System and Effects of Clonidine and Guanfacine 158
ABSTRACT 158
KEYWORDS 158
INTRODUCTION 158
MATERIALS AND METHODS 159
RESULTS 160
DISCUSSION 165
ACKNOWLEDGEMENT 166
REFERENCES 166
Chapter 15. The Role of Glutamate Decarboxylase and GABA Transaminase in Post Mortem GABA Increases in Discrete Regions of Rat Brain 168
ABSTRACT 168
KEYWORDS 168
INTRODUCTION 169
MATERIALS AND METHODS 169
RESULTS 170
DISCUSSION 174
REFERENCES 174
Chapter 16. Changes in the Levels of Cyclic Nucleotides and Monoamines in Rat Brain During the Sleep–Wakefulness Cycle: Application of the Microwave Fixation Method to Sleep Research 176
ABSTRACT 176
KEYWORDS 176
INTRODUCTION 177
MATERIALS AND METHODS 177
RESULTS 179
DISCUSSION 182
REFERENCES 186
Chapter 17. Study of Regional Blood Volume in the Brain of the Mouse Using 250 msec Coagulation Following Administration of a Dihydrogenated Ergot Alkaloid (Hydergine) 190
ABSTRACT 190
KEYWORDS 190
INTRODUCTION 190
METHODS 192
RESULTS 192
DISCUSSION 193
ACKNOWLEDGEMENT 195
REFERENCES 195
AUTHOR INDEX 198
SUBJECT INDEX 200