Human Embryonic Stem Cells (eBook)

Stephen Sullivan: Trinity College, Dublin, Ireland Kevin Eggan: Harvard University, Cambridge, MA, USA Chad A. Cowan: Harvard University, Cambridge, MA, USA

Foreword. Davor Solter

Preface.

Section 1: Obtaining and culturing human embryonic stem
cells.

1 Organization and good aseptic technique in the human embryonic
stem cell laboratory.

Minal J Patel, Emma L Stephenson, and Stephen L Minger

2 Sourcing human embryonic stem cell lines.

Glyn Stacey

3 Human embryo culture for human embryonic stem cell
derivation.

R. Douglas Powers and Jeannine Witmyer

4 Derivation of human embryonic stem cell lines.

Stephen Sullivan, Dieter Egli, Hidenori Akutsu, Douglas A. Melton,
Kevin Eggan and Chad A. Cowan

5 Standard culture of human embryonic stem cells.

Jeremy M. Crook, Rachel Horne, and Alan Colman

6 Culture of human embryonic stem cells in chemically defined
conditions.

Julie Hsu Clark and Sheng Ding

Section 2: Characterization of undifferentiated embryonic stem
cells.

7 Phenotypic analysis of human embryonic stem cells.

Jonathan S. Draper, Cheryle A. Séguin and Peter W.
Andrews

8 Genetic and epigenetic analysis of human embryonic stem
cells.

Laurie A. Boyer, Rudolf Jaenisch, and Maisam Mitalipova

Section 3: Manipulation of human embryonic stem cells.

9 In vivo differentiation of human embryonic stem
cells.

Scott A. Noggle, Francesca M. Spagnoli, Ali H. Brivanlou

10 In vitro differentiation of human embryonic stem
cells.

A. Henry Sathananthan and Alan Trounson

11 Differentiation of human embryonic stem cells into
extraembryonic cell types.

Cheryle A. Séguin and Jonathan S. Draper

12 Directed differentiation of human embryonic stem cells
into:

(a) Early endoderm cells.

Kenji Osafune, Alice E. Chen and Douglas A. Melton

(b) Hepatic cells.

Neta Lavon and Nissim Benvenisty

(c) Pancreatic cells.

Hiram Chipperfield

13 Directed differentiation of human embryonic stem cells
into:

(a) Cardiomyocytes.

Christine Mummery, Robert Passier and Chris Denning

(b) Endothelial cells.

Carrie Soukup, Shulamit Levenberg and Ondine Cleaver

(c) Osteogenic cells.

Jeffrey M. Karp, Alborz Mahdavi, Lino S. Ferreira, Ali
Khademhosseini and Robert Langer

(d) Hematopoietic in vivo repopulating cells.

Shannon McKinney-Freeman, Thorsten M. Schlaeger and George Q.
Daley

(e) Lymphocytes.

Petter S. Woll and Dan S. Kaufman

(f) Myeloid cells.

Chantal Cerdan and Mickie Bhatia

14 Directed differentiation of human embryonic stem cells
into:

(a) Forebrain neurons.

Emily A. Davis and Lawrence S.B. Goldstein

(b) Dopaminergic neuron.

Jan Pruszak and Ole Isacson

(c) Spinal motor neurons.

Bao-Yang Hu and Su-Chun Zhang

15 (a) Gene targeting: knock out and knock in by
homologous recombination.

Thomas P. Zwaka

(b) RNA interference in human embryonic stem cells.

M. William Lensch, Asmin Tulpule and Holm Zaehres

(c) Generation of gene reporters using bacterial artificial
chromosome recombineering. Andrew J. Washkowitz and David A.
Shaywitz

Afterword. Azim Surani

Index.

"A highly readable and useful book ... .[It] will help many to
navigate the uncharted waters of human embryonic stem cell
biology." (The British Society for Cell Biology
Newsletter, Autumn, 2008)

"[It] is a valuable resource for seasoned and novice
researchers; it would make an excellent addition to the reference
collection of any medical library or research laboratory."
(American Medical Association, September 2008)

"...the imaginative solutions in this book can inspire us to get
past our most frustrating limitations." (Cell Stem Cell,
September 2007)

"...the richness in the details of each protocol presented will
certainly encourage more scientists to begin studies of Human ES
cells..." (Regenerative Medicine, 2007)

"In this fast-moving field, this [handbook] will help drive
advances of more and more researchers."
(Differentiation, 2007)

"A highly readable and useful book ... [This book] will help
many to navigate the uncharted waters of human embryonic stem cell
biology." (British Society for Cell Biology, Autumn,
2008)