Fluorescence Spectroscopy in Biology (eBook)

Series Editor 5
Preface 6
Contents 8
Contributors 16
Part 1 Fluorescence Spectroscopy: Basics and Advanced Approaches 19
1 Basics of Fluorescence Spectroscopy in Biosciences 20
2 Pulse and Phase Fluorometries: An Objective Comparison 47
3 Non- Exponential Fluorescence of Electronically Coupled Donors Contains Distance Information 66
4 Fluorescence Nanotomography: Recent Progress, Constraints and Opportunities 73
5 Solvent Relaxation as a Tool for Probing Micro- Polarity and - Fluidity 88
6 Total Internal Reflection Fluorescence Microscopy: Applications in Biophysics 96
7 Single Molecule Spectroscopy: Basics and Applications 121
Part 2 Application of Fluorescence Spectroscopy to Biological Membranes 148
8 Raft Microdomains in Model Membranes as Revealed by Fluorescence Quenching 149
9 The Lateral Structure of Lipid Membranes as Seen by Fluorescence Microscopy 166
Part 3 Application of Fluorescence Spectroscopy to Protein Studies 176
10 Protein Dynamics and Protein Folding Dynamics Revealed by Time- Resolved Fluorescence 177
11 Time- Resolved Fluorescence and Two- Photon FCS Investigation of the Interaction of HIV- 1 Nucleocapsid Protein with Hairpin Loop Oligonucleotides 194
Part 4 Application of Fluorescence Spectroscopy to DNA and Drug Delivery 212
12 Fluorescence Techniques in Non- Viral Gene Therapy 213
13 Fluorescence Applications in Targeted Drug Delivery 241
Part 5 Fluorescence Spectroscopy in cells: FCS and Quantum Dots 255
14 Fluorescence Correlation Spectroscopy in Cell Biology 256
15 Fluorescent Quantum Dots: Properties and Applications 274
16 Heat Stress of Cancer Cells: Fluorescence Imaging of Structural Changes with Quantum Dots 605 and Alexa 488 286
Sachverzeichnis 299