Antigen Retrieval Immunohistochemistry Based Research and Diagnostics

Shan-Rong Shi, MD, is professor of clinical pathology at the Keck School of Medicine of the University of Southern California. He is the Associate Editor of The Journal of Histochemistry and cytochemistry, he is on the Editoral Board of Applied Immunohistochemistry and Molecular Morphology and is also on the Overseas Editorial Board of the Chinese Journal of Pathology. Dr. Shi is the ineventor of the antigen retrieval (AR) technique. Clive R. Taylor, MD, PhD , is Senior Associate Dean, Professor and Chairman of Pathology at the Keck School of Medicine. Dr. Taylor is Editor in Chief of Applied Immunohistochemistry and Molecular Morphology. Dr. Taylor is a world renowned expert in immunohistochemistry and clinical pathology.

PREFACE. CONTRIBUTORS. PART I: RECENT ADVANCES IN ANTIGEN RETRIEVAL TECHNIQUES AND ITS APPLICATION. 1 STANDARDIZATION OF ANTIGEN RETRIEVAL TECHNIQUES BASED ON THE TEST BATTERY APPROACH ( Shan-Rong Shi and Clive R. Taylor ). 2 EXTENDED APPLICATION OF ANTIGEN RETRIEVAL TECHNIQUE IN IMMUNOHISTOCHEMISTRY AND IN SITU HYBRIDIZATION ( Shan-Rong Shi and Clive R. Taylor ). 3 EXTRACTION OF DNA/RNA FROM FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUE BASED ON THE ANTIGEN RETRIEVAL PRINCIPLE ( Shan-Rong Shi and Clive R. Taylor ). PART II: STANDARDIZATION OF IMMUNOHISTOCHEMISTRY. 4 KEY ISSUES AND STRATEGIES OF STANDARDIZATION FOR QUANTIFIABLE IMMUNOHISTOCHEMISTRY ( Shan-Rong Shi, Kevin A. Roth, and Clive R. Taylor ). 5 STANDARDIZATION OF IMMUNOHISTOCHEMISTRY BASED ON ANTIGEN RETRIEVAL TECHNIQUE ( Shan-Rong Shi and Clive R. Taylor ). 6 STANDARD REFERENCE MATERIAL: CELL LINE DEVELOPMENT AND USE OF REFERENCE CELL LINES AS STANDARDS FOR EXTERNAL QUALITY ASSURANCE OF HER2 IHC AND ISH TESTING ( Bharat Jasani, Vicky Reid, Colin Tristram, Jeremy Walker, Paul Scorer, Michael Morgan, John Bartlett, Merdol Ibrahim, and Keith Miller ). 7 PEPTIDES AS IMMUNOHISTOCHEMISTRY CONTROLS ( Steven A. Bogen and Seshi R. Sompuram ). 8 STANDARD REFERENCE MATERIAL: PROTEINEMBEDDING TECHNIQUE AND DESIGN OF BAR CODE ( Shan-Rong Shi, Jiang Gu, and Clive R. Taylor ). 9 THE PROS AND CONS OF AUTOMATION FOR IMMUNOHISTOCHEMISTRY FROM THE PROSPECTIVE OF THE PATHOLOGY LABORATORY ( David G. Hicks and Loralee McMahon ). 10 IMAGE ANALYSIS IN IMMUNOHISTOCHEMISTRY ( Alton D. Floyd ). PART III TISSUE/CELL SAMPLE PREPARATION. 11 TISSUE CELL SAMPLE PREPARATION: LESSONS FROM THE ANTIGEN RETRIEVAL TECHNIQUE ( Shan-Rong Shi and Clive R. Taylor ). 12 MECHANISMS OF ACTION AND PROPER USE OF COMMON FIXATIVES ( Richard W. Dapson ). 13 CELL SAMPLE PREPARATION FOR CLINICAL CYTOPATHOLOGY: CURRENT STATUS AND FUTURE DEVELOPMENT ( Yan Shi and Patricia G. Wasserman ). 14 DESIGN OF A TISSUE SURROGATE TO EXAMINE ACCURACY OF PROTEOMIC ANALYSIS ( Carol B. Fowler, Jeffrey T. Mason, and Timothy J. O'leary ). PART IV: MOLECULAR MECHANISM OF ANTIGEN RETRIEVAL TECHNIQUE. 15 STUDY OF FORMALIN FIXATION AND HEAT-INDUCED ANTIGEN RETRIEVAL ( Jeffrey T. Mason, Carol B. Fowler, and Timothy J. O'leary ). 16 A LINEAR EPITOPES MODEL OF ANTIGEN RETRIEVAL ( Steven A. Bogen and Seshi R. Sompuram ). 17 PH OR IONIC STRENGTH OF ANTIGEN RETRIEVAL SOLUTION: A POTENTIAL ROLE FOR REFOLDING DURING HEAT TREATMENT ( Shuji Yamashita ). 18 COMMENTARY: FUTURE DIRECTIONS ( Timothy J. O'Leary, Carol B. Fowler, David L. Evers, Robert E. Cunningham, and Jeffrey T. Mason ). PART V: PROTEOMIC ANALYSIS OF PROTEIN EXTRACTED FROM TISSUE/CELLS. 19 TECHNIQUES OF PROTEIN EXTRACTION FROM FFPE TISSUE/CELLS FOR MASS SPECTROMETRY ( Carol B. Fowler, Timothy J. O'Leary, and Jeffrey T. Mason ). 20 APPLICATION OF SHOTGUN PROTEOMICS TO FORMALIN-FIXED AND PARAFFIN-EMBEDDED TISSUES ( Brian M. Balgley ). 21 VISUALIZING PROTEIN MAPS IN TISSUE ( Masahiro Mukai and Mitsutoshi Setou ). 22 SYMBIOSIS OF IMMUNOHISTOCHEMISTRY AND PROTEOMICS: MARCHING TO A NEW ERA ( Shan-Rong Shi, Brian M. Balgley, and Clive R. Taylor ). APPENDIX RELATED LABORATORY PROTOCOLS. INDEX.