Laser Capture Microdissection
Humana Press Inc. (Verlag)
978-1-61779-162-8 (ISBN)
Authoritative and cutting-edge, Laser Capture Microdissection: Methods and Protocols, Second Edition is an ideal resource for researchers striving to move forward our understanding of normal physiology and pathology.
Laser Capture Microdissection: Methods and Applications.- Laser Microdissection for Gene Expression Profiling.- Gene Expression Using the PALM System.- Immuno-Guided Microdissection Techniques.- Optimized RNA Extraction from Non-Deparaffinized, Laser-Microdissected Material.- Laser Capture Microdissection for Analysis of Gene Expression in Formalin-Fixed Paraffin-Embedded Tissue.- MicroRNA Profiling Using RNA from Microdissected Immunostained Tissue.- Profiling Solid Tumor Heterogeneity by LCM and Biological MS of Fresh Frozen Tissue Sections.- Amplification Testing in Breast Cancer by Multiplex Ligation-Dependent Probe Amplification of Microdissected Tissue.- Detection and Quantification of MicroRNAs in Laser Microdissected Formalin-Fixed Paraffin-Embedded Breast Cancer Tissues.- Laser Capture Microdissection Applications in Breast Cancer Proteomics.- Proteomic Analysis of Laser Microdissected Ovarian Cancer Tissue with SELDI-TOF MS.- LCM Assisted Biomarker Discovery from Archival Neoplastic Gastrointestinal Tissues.- Purification of Diseased Cells from Barrett’s Esophagus and Related Lesions by Laser Capture Microdissection.- Laser Microdissection of Intestinal Epithelial Cells and Downstream Analysis.- Application of Laser Microdissection and Quantitative PCR to Assess the Response of Oesophageal Cancer to Neoadjuvant Chemo-Radiotherapy.- Oligonucleotide Microarray Expression Profiling of Contrasting Invasive Phenotypes in Colorectal Cancer.- Evaluation of Gastrointestinal mtDNA Depletion in Mitochondrial Neurogastrointestinal Encephalomyopathy (MNGIE).- Laser Microdissection for Gene Expression Study of Hepatocellular Carcinomas Arising in Cirrhotic and Non-Cirrhotic Livers.- Laser Capture Microdissection of Pancreatic Ductal Adeno-Carcinoma Cells to AnalyzeEzH2 by Western Blot Analysis.- Laser-Capture Microdissection of Renal Tubule Cells and Linear Amplification of RNA for Microarray Profiling and Real-Time PCR.- Subcellular Renal Proximal Tubular Mitochondrial Toxicity with Tenofovir Treatment.- Application of Laser Capture Microdissection to Study Renal Carcinogenesis.- Laser Capture Microdissection and Transcriptional Profiling in Archival FFPE Tissue in Prostate Cancer.- Quantitative Analysis of the Enzymes Associated with 5-Fluorouracil Metabolism in Prostate Cancer Biopsies.- Microdissection of Gonadal Tissues for Gene Expression Analyses.- Duplex Real Time PCR Assay for Quantifying Mitochondrial DNA Deletions in Laser Microdissected Single Spiral Ganglion Cells.- Neuronal Type-Specific Gene Expression Profiling and Laser-Capture Microdissection.- Region-Specific In Situ Hybridization-Guided Laser Capture Microdissection on Postmortem Human Brain Tissue Coupled with Gene Expression Quantification.- UV-Laser Microdissection and mRNA Expression Analysis of Individual Neurons from Post Mortem Parkinson’s Disease Brains.- Transcriptome Profiling of Murine Spinal Neurulation Using Laser Capture Microdissection and High-Density Oligonucleotide Microarrays.- Probing the CNS Microvascular Endothelium by Immune-Guided Laser Capture Microdissection Coupled to Quantitative RT-PCR.- Laser Capture Microdissection for Factor VIII-Expressing Endothelial Cells in Cancer Tissues.- Laser Capture Microdissection and Analysis of Liver Endothelial Cells from Patients with Budd Chiari Syndrome.- Laser Capture Microdissection of Hyperlipidemic/ApoE-/- Mouse Aorta Atherosclerosis.- Gene Expression Profiling in Laser Microdissected Bone Marrow Megakaryocytes.- Specific RNA Collection from the Rat Endolymphatic Sac by LaserCapture Microdissection (LCM): LCM of Very Small Organ Surrounded by Bony Tissue.- The Use of Laser Capture Microdissection on Adult Human Articular Cartilage for Gene Expression Analysis.- Laser Capture Microdissection of Developing Barley Seeds and cDNA Array Analysis of Selected Tissues.- Quantitative RT-PCR Gene Expression Analysis of a Laser Microdissected Placenta: An Approach to Study Preeclampsia.
Reihe/Serie | Methods in Molecular Biology ; 755 |
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Zusatzinfo | 1 Illustrations, color; 96 Illustrations, black and white; XVII, 493 p. 97 illus., 1 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Technik ► Umwelttechnik / Biotechnologie | |
Schlagworte | Downstream applications • Gene expression analysis • microarrays • Molecular analysis • Molecular technologies • PCR • Proteomics • Tissue-based laser microdissection |
ISBN-10 | 1-61779-162-8 / 1617791628 |
ISBN-13 | 978-1-61779-162-8 / 9781617791628 |
Zustand | Neuware |
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