Protein Targeting Protocols

Bacterial Systems.- Localization and Mobility of Bacterial Proteins by Confocal Microscopy and Fluorescence Recovery After Photobleaching.- Membrane Protein Insertion and Secretion in Bacteria.- Identification of Autotransporter Proteins Secreted by Type V Secretion Systems in Gram-Negative Bacteria.- Periplasmic Targeting of Recombinant Proteins in Escherichia coli.- In Vitro Analysis of the Bacterial Twin-Arginine-Dependent Protein Export.- Eukaryotic Systems.- Nuclear Import Properties of the Sex-Determining Factor SRY.- The Mitochondrial Machinery for Import of Precursor Proteins.- A Genetic Method to Identify Mitochondrial Proteins in Living Mammalian Cells.- In Vitro and In Vivo Methods to Study Protein Import Into Plant Mitochondria.- Protein Targeting to Mitochondria of Saccharomyces cerevisiae and Neurospora crassa.- A Mitosome Purification Protocol Based on Percoll Density Gradients and Its Use in Validating the Mitosomal Nature of Entamoeba histolytica Mitochondrial Hsp70.- The Chloroplast Protein Import Machinery: A Review.- Import of Plastid Precursor Proteins Into Pea Chloroplasts.- Protein Targeting in ‘‘Secondary’’ or ‘‘Complex’’ Chloroplasts.- Protein Trafficking to the Complex Chloroplasts of Euglena.- Fluorescent Protein Fusions for Protein Localization in Plants.- Genetic Transformation.- Identification of Proteins Targeted Into the Endoplasmic Reticulum by cDNA Library Screening.- Trafficking Through the Early Secretory Pathway of Mammalian Cells.- Studying Protein Export From the Endoplasmic Reticulum in Plants.- Imaging the Golgi Apparatus in Living Mitotic Cells.- Evaluating Yeast Biosynthetic Vacuolar Transport.- Targeting to Lysosomes in Mammalian Cells.- Monitoring Autophagy in Yeast.- Protein Targeting to Yeast Peroxisomes.-Expression, Localization, and Topology of Fluorescently Tagged Plasma Membrane-Targeted Transmembrane Proteins.- General Methods.- Intracellular Protein Localization by Immunoelectron Microscopy.- Separation of Proteins by Blue Native Electrophoresis.- Computational Prediction of Subcellular Localization.- Phylogenetic Analysis to Uncover Organellar Origins of Nuclear-Encoded Genes.