Diagnostic and Therapeutic Antibodies

The Antibody Molecule.- Polyclonal and Monoclonal Antibodies.- Engineering Antibody Molecules.- Phage Display Technology.- Antibodies in Medicine.- Prospects for the Application of Antibodies in Medicine.- Antibodies for Neoplastic Disease.- The Application of Monoclonal Antibodies in the Treatment of Lymphoma.- Antibodies for Inflammatory Disease.- Antibodies for Inflammatory Disease.- Antibodies for Transplantation.- Antibody-Based Therapies in Infectious Diseases.- Antibodies in Nuclear Medicine.- Animal Models for Tumor Localization.- Antibodies for Immunoassays.- Ethics and Industry.- Intellectual Property.- From Laboratory to Clinic.- Production and Purification.- Production of Monoclonal Antibodies.- Purification of Monoclonal Antibodies Using Protein A/G.- Preparation of Monoclonal Antibodies Using Ion Exchange Chromatography.- Quality Control of Raw Materials.- Cell Banks and Stability of Antibody Production.- Measurement of Endotoxin.- Aseptic Vial Filling.- Measurement of Antibody Concentrations by Hemagglutination.- Modification of Antibodies.- Enzymatic Digestion of Monoclonal Antibodies.- How to Make Bispecific Antibodies.- Radiolabeling of Monoclonal Antibodies.- Determination of the Immunoreactivity of Radiolabeled Monoclonal Antibodies.- Use of Biosensors to Measure the Kinetics of Antibody-Antigen Interactions.- Application of Antibodies in Vitro.- How to Set Up an ELISA.- Measurement of HAMA and Anti-Idiotypic Antibodies.- SDS-PAGE and Western Blotting.- Flow Cytometric Analysis.- Immunocytochemistry.- Immunolabeling for Electron Microscopy.- Antibody Engineering.- PCR of the V Region.- Phage Display Technology.

"...a welcome addition to the clinician's medical library....written by experts in the field and cover a broad selection of issues, ranging from the basic structure of antibodies to intellectual property rights....Although the chapters can stand independently, the editors have made a major effort to cross-index them by giving references to related material in other chapters....this is a comprehensive book that should be useful to anyone interested in the use of antibodies for therapy, diagnosis, or laboratory investigation. As someone who is currently involved in the development of an antibody-based therapy, I found this book to be quite complete and well written. In many ways it can serve as a manual for the development and use of antibody reagents. The chapters are liberally sprinkled with welcome advice.... For clinicians, the book can provide a handy reference for the many types of antibody reagents available for clinical use, as well as information on serological assays. This book fills an important niche in the literature on antibody-based therapies, at a time when reagents are finding their way into clinical use in ever-increasing numbers." - Clinical Infectious Diseases