Plant Gametogenesis

Measurement of flower metabolite concentrations using Gas-Chromatography Mass Spectrometry and High-Performance Liquid Chromatography Mass Spectrometry.- Proteomic-, phosphoproteomic- and Acetylomic-based mass spectrometry to identify tissue-specific protein complexes and phosphorylation in plant gametogenesis.- Identifying small RNAs and analyzing their association with gene expression using isolated Arabidopsis male meiocytes.- Genotyping-by-sequencing to map meiotic crossovers in plants.- Interrogating global chromatin interaction network by high-throughput chromosome conformation capture (Hi-C) in plants.- Cytogenetic techniques for analysing meiosis in hexaploidy bread wheat.- Super-resolution chromatin visualisation by combined fluorescence in situ hybridisation and Structured Illumination Microscopy in Solanum lycopersicum.- Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy.- Bimolecular Fluorescence Complementation to Test for Protein-Protein Interactions and To Uncover Regulatory Mechanisms During Gametogenesis.- Quantifying meiotic crossover recombination in Arabidopsis lines expressing fluorescent reporters in seeds using SeedScoring pipeline for CellProfile.- Generation of mutants by combined treatment of physical and chemical mutagens in rice.- Generation of mutant plants by Gamma Ray exposure and development of low-cost TILLING population in chickpea (Cicer arietinum L.).- Efficient generation of CRISPR/Cas9-based mutants supported by fluorescent seed selection in different Arabidopsis accessions.- Generation of deletion lines in Allohexaploid Bread Wheat.- Post-transcriptional Gene Silencing Using Virus-Induced Gene Silencing to Study Plant Gametogenesis in Tomato.- Protocols for in vivo doubled haploid (DH) technology in maize breeding: From haploid inducer Development to Haploid Genome Doubling.- Estimation of heterografting associated DNA methylation changes in tree crops by MSAP analysis.- In vitro fertilization system using wheat gametes by electric fusion.- High-throughput fluorescent pollen tetrad analysis using DeepTetrad.- Barley (Hordeum vulgare) anther and meiocyte RNA Sequencing: Mapping Sequencing reads and downstream data analyses.- Profiling m6A RNA modifications in low amounts of plant cells using Maize Meiocytes.- Assembling Plant Genomes with Long-Read Sequencing.- Mapping-by-sequencing of point and insertional mutations with Easymap.- Characterization of the different levels of variation in 45S rRNA genes.