Microbial Systems Biology

Parallel Accelerator and Molecular Mass Spectrometry Measurement of Carbon-14 Labeled Analytes.- Fast sampling of the cellular metabolome.- Investigation of Protein-Lipid Interactions Using Native Mass Spectrometry.- Western Blot Processing Optimization: The Perfect Blot.- FISHing on a Budget.- NanoSIP: NanoSIMS Applications for Microbial Biology.- Construction of Metatranscriptomic Libraries for 5' end Sequencing of rRNAs for Microbiome Research.- Computational Approaches for Designing Highly Specific and Efficient sgRNAs.- Complex Network Analysis in Microbial Systems: Theory and Examples.- Prokaryotic Genome Annotation.- Functional Annotation from Structural Homology.- Metabolic Modeling with MetaFlux.- Application of the Metabolic Modeling Pipeline in KBase to Categorize Reactions, Predict Essential Genes, And Predict Pathways in an Isolate Genome.- Curating COBRA Models of Microbial Metabolism.- A Beginner’s Guide to the COBRA Toolbox.- Rules of Engagement: A Guide to Developing Agent-Based Models.