One-Step Generation of a Drug-Releasing Microarray for High-Throughput Small-Volume Bioassays

Seo Woo Song (Autor)

Buch | Hardcover

52 Seiten

2019 | 1st ed. 2019
Springer Verlag, Singapore
978-981-13-8093-8 (ISBN)

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This thesis demonstrates a technology that enables pipetting-free high-throughput screening (HTS) on a miniaturized platform, eliminating the need for thousands of one-by-one pipetting and conventional liquid handling systems. This platform enhances accessibility to HTS and enables HTS to be used in small-to-medium scale laboratories. In addition, it allows large-scale combinatorial screening with a small number of valuable cells, such as patients’ primary cancer cells. This technique will have a high impact for widespread use of HTS in the era of personalized medicine.
In this thesis, the author firstly describes the need and concept of ‘partipetting’ for pipetting-free HTS platform. It is realized by the one-step pipetting and self-assembly of encoded drug-laden microparticles (DLPs) on the microwells. Next, the technical implementations required for the platform demonstration are described. It includes preparation of encoded DLPs, plastic chip fabrication, andrealization of automated system. Lastly, screening of sequential drug combinations using this platform is demonstrated. This shows the potential of the proposed technology for various applications.

Seo Woo Song earned PhD degree in the department of electrical and computer engineering from Seoul National University, Republic of Korea in 2019. His research interests are lab on a chip, bioMEMS, and micro-robotics.

Abstract.- Table of Contents.- List of Tables.- List of Figures.- Chapter 1. Introduction.- 1.1. High-Throughput Small-Volume Bioassays.- 1.2. Developmental Goal for the ‘Pipetting-Free’ HTS Platforms.- 1.3. Main Concept: One-Step Generation of a Drug-Releasing Microarray-on-a-Chip by Self-Assembly of Drug-Laden Microparticles (DLPs).- Chapter 2. System Development.- 2.1. Sealing-Film Assisted Seeding Method for Saving Cell Consumptions.- 2.2. Chip and Jig Development.- 2.3. Preparation of DLPs Library.- 2.4. Decoding Microparticles.- 2.5. Statistical Analysis for Duplications.- Chapter 3. Application: Screening of Sequential Drug Combinations.- 3.1. Therapeutic Benefit of Sequential Drug Combination Based on Rewiring of Intracellular Pathways.- 3.2. Screening of Sequential Drug Combination Using a Partipetting Platform.- 3.3. Proof-of-Concept: Sequential Combinatorial Cell Staining Assay by Replacement of the Drug Chip.- 3.4. Screening of Sequential Combinatorial Drugs with EGFR Inhibitor Followed by Genotoxin against Triple Negative Breast Cancer (TNBC).- Chapter 4. Conclusion and Discussion.- Bibliography.

Erscheinungsdatum	04.06.2019
Reihe/Serie	Springer Theses
Zusatzinfo	40 Illustrations, color; 6 Illustrations, black and white; XXI, 52 p. 46 illus., 40 illus. in color.
Verlagsort	Singapore
Sprache	englisch
Maße	155 x 235 mm
Themenwelt	Mathematik / Informatik ► Informatik ► Theorie / Studium
	Medizin / Pharmazie ► Physiotherapie / Ergotherapie ► Orthopädie
	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
	Naturwissenschaften ► Biologie
	Naturwissenschaften ► Physik / Astronomie ► Thermodynamik
	Technik ► Medizintechnik
Schlagworte	Drug releasing • Encoded drug -laden microparticles • High-throughput screening • microarray • Microarray-On-A-Chip • Microparticles
ISBN-10	981-13-8093-7 / 9811380937
ISBN-13	978-981-13-8093-8 / 9789811380938
Zustand	Neuware