Unravelling Single Cell Genomics (eBook)

After graduating from the Ecole Polytechnique, Nathalie Bontoux completed her MSc and PhD at the Paris VI University. She also carried out graduate research at the Laboratory of Photonics and Nanostructures, in Marcoussis and the Laboratory of Neurobiology at the ESPCI in Paris. In addition to her research, she has also held teaching posts at the LycÚe Saint Louis, Ecole Polytechnique and Orsay University. Currently, she is working as a product specialist on bioconsumables, including Lab-on-a-Chip kits, at Agilent Technologies. Marie-Claude Potier is recognized as a world expert in Down's syndrome and gene expression. She started as a Staff Scientist CNRS in 1991 before completing a PhD in Molecular Neuropharmacology and three years postdoctoral research at the Laboratory of Molecular Biology of the MRC in Cambridge. Her work focuses on the characterization of Down's syndrome phenotypes and the development of therapeutic approaches in mouse models. For the past decade, she has also been working on high throughput gene expression technologies using microarrays which have revealed gene expression modifications in Down's syndrome. More recently, using microfluidics, she has been involved in the development of a breakthrough method for gene expression profiling in single cells that has been used to study specific neuronal populations in the brain. For the past 6 years, she has led a research team and is currently the Vice Director of the CNRS unit.

Introduction Basic concepts in molecular and cell biology Single cell analysis: a tool to understand basic biological mechanisms and concepts State-of-the-art single cell genomics and proteomics Introduction to MEMS and microfluidic devices State-of-the-art microfluidic systems for genomics and proteomics A concrete case: a microfluidic device for single cell whole transcriptome analysis Pushing the limits: manipulating cell-like volumes and detecting single copies Conclusion