Pyrosequencing (eBook)

Part I: Introduction1. The History of PyrosequencingÒPål Nyrén
 
2. PyroMark® Instruments, Chemistry, and Software for Pyrosequencing® Analysis
Martin Kreutz, Gerald Schock, Julia Kaiser, Norbert Hochstein, and Ralf Peist
 
3. Software-Based Pyrogram® Evaluation
Guoli Chen, Matthew T. Olson, and James R. Eshleman
 
4. Quantitative Validation and Quality Control of Pyrosequencing® Assays
Ulrich Lehmann
 
Part II: Analysis of Genetic Variation
5. Extended KRAS and NRAS Mutation Profiling by Pyrosequencing®
Andreas Jung
 
6. Universal BRAF State Detection by the Pyrosequencing®-based U‑BRAFV600 Assay
Alexander Skorokhod
 
7. Pyrosequencing®-based Identification of Low Frequency Mutations Enriched through Enhanced-ice-COLD-PCR
Alexandre How-Kit and Jörg Tos
 
8. Analysis of Mutational Hotspots in Routinely Processed Bone Marrow Trephines by Pyrosequencing®
Stephan Bartels and  Ulrich Lehmann
 
9. Analysis of Copy Number Variation By Pyrosequencing® using Paralogous Sequences
Marianne Kristiansen Kringen
 
10. Prenatal Diagnosis of Chromosomal Aneuploidies by Quantitative Pyrosequencing®
Hui Ye, Haiping Wu, Yunlong Liu, Bingjie Zou, Tomoharu Kajiyama,  Hideki Kambara, and Guohua Zhou
 
11. HLA-B and HLA-C Supratyping by Pyrosequencing®
Irene Vanni, Elisabetta Ugolotti, Patrizia Larghero, and Roberto Biassoni
 
12. Allele Quantification Pyrosequencing® at Designated SNP sites to Detect Allelic Expression Imbalance and Loss-of-heterozygosity
Chau-To Kwok and Megan P. Hitchins
 
Part III: Analysis of DNA Methylation Patterns
13. Quantitative DNA Methylation Analysis by Pyrosequencing®
Jessica Roessler and Ulrich Lehmann 
14. Quantitative Methylation Analysis of the PCDHB Gene Cluster
Barbara Banelli and Massimo Romani
 
15. Assessment of Changes in global DNA Methylation Levels by Pyrosequencing® of Repetitive Elements
Ali M. Tabish, Andrea A. Baccarelli, Lode Godderis, Timothy M. Barrow, Peter Hoet1 and Hyang-Min Byun
 
16. Global Analysis of DNA 5-methylcytosine using the Luminometric
Methylation Assay, LUMA
Karin Luttropp, Louise K Sjöholm, and Tomas J Ekström
 
17. Limiting Dilution Bisulfite Pyrosequencing®: a Method for Methylation Analysis of Individual DNA Molecules in a Single or a Few Cells
Nady El Hajj, Juliane Kuhtz, and Thomas Haaf
 
18. Detection of Loss of Imprinting by Pyrosequencing®
Silvia Tabano, Eleonora Bonaparte,  and Monica Miozzo
 
19. Analysis of DNA Methylation Patterns in Single Blastocysts by Pyrosequencing®
John Huntriss, Kathryn Woodfine, Joanna E Huddleston, Adele Murrell, and  Helen Mary Picton
 
20. Allele-specific DNA methylation detection by Pyrosequencing®
Lasse Sommer Kristensen, Jens Vilstrup Johansen, and Kirsten Grønbæk
 
21. SNP-based Quantification of Allele-Specific DNA Methylation Patterns by Pyrosequencing®
Florence Busato and Jörg Tost
 
22. DNA Methylation Analysis of ChIP Products at Single Nucleotide Resolution byPyrosequencing®
Céline Moison, Fanny Assemat, Antoine Daunay, Paola B Arimondo, and Jörg Tost
 
Part IV: Bacterial Typing and Identification
23. Multiplex Pyrosequencing®: Simultaneous Genotyping Based on SNPs from Distant Genomic Regions
Piotr Wojciech Dabrowski, Kati Bourquain, and Andreas Nitsche
 
24. Detection of Drug-Resistant Mycobacterium tuberculosis
Anna Engström and Pontus Juréen
 
25. Application of Pyrosequencing® in Food Biodefense
Kingsley Kwaku Amoako
 
Part V: Forensics
26. Forensic Analysis of Mitochondrial and Autosomal Markers using Pyrosequencing®
Magdalena M. Buś, Hanna Edlund, and Marie Allen
 
27. Tissue-specific DNA Methylation Patterns in Forensic Samples Detected By Pyrosequencing®
Joana Antunes, Kuppareddi Balamurugan, George Duncan, and Bruce McCord