Mitochondrial DNA
Humana Press Inc. (Verlag)
978-1-4939-3039-5 (ISBN)
Authoritative and practical, Mitochondrial DNA: Methods and Protocols, Third Edition will be useful not only for mitochondrial researchers but also for scientists studying human diseases where mitochondrial DNA variation has been recognized as an important pathogenic factor, including cancer and neurodegeneration.
Comprehensive Mitochondrial Genome Analysis By Massively Parallel Sequencing.- Analysis Of Translesion DNA Synthesis By The Mitochondrial DNA Polymerase g.- Quantification Of DNA Damage By Real-Time Qpcr.- Quantitation Of Mitochondrial DNA Deletions Via Restriction Digestion/Long-Range Single Molecule PCR.- A Single-Cell Resolution Imaging Protocol Of Mitochondrial DNA Dynamics In Physiopathology, Mtrip, Which Also Evaluates Sublethal Cytotoxicity.- Scalable Isolation Of Mammalian Mitochondria For Nucleic Acid And Nucleoid Analysis.- Tracking Mitochondrial DNA In Situ.- Analysis Of Replicating Mitochondrial DNA By In Organello Labeling And Two-Dimensional Agarose Gel Electrophoresis.- Translation And Assembly Of Radiolabelled Mitochondrial DNA-Encoded Protein Subunits From Cultured Cells And Isolated Mitochondria.- Analysis Of Mitochondrial DNA Copy Number And Its Regulation Through DNA Methylation Of POLGA.- Engineered Mtzfns For Manipulation Of Human Mitochondrial DNA Heteroplasm.- Generation Of Xenomitochondrial Embryonic Stem Cells For The Production Of Live Xenomitochondrial Mice.- Import Of Fluorescent RNA Into Mitochondria Of Living Cells.- Purification And Comparative Assay Of The Human Mitochondrial Replicative DNA Helicase.- Expression And Purification Of Mitochondrial RNA Polymerase And Transcription Factor A From Drosophila Melanogaster.- Purification And Comparative Assay Of Human Mitochondrial Single-Stranded DNA-Binding Protein.- Biolayer Interferometry: A Novel Method To Elucidate Protein- Protein And Protein- DNA Interactions In The Mitochondrial DNA Replisome.
Reihe/Serie | Methods in Molecular Biology ; 1351 |
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Zusatzinfo | XI, 234 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Informatik ► Weitere Themen ► Bioinformatik |
Medizin / Pharmazie ► Medizinische Fachgebiete | |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Schlagworte | mitochondria • mitochondrial DNA analysis • mitochondrial DNA copy number • mitochondrial DNA damage • mitochondrial DNA-encoded protein translation • mitochondrial DNA replication • Mitochondrial genome • mutations • next-generation massive parallel sequencing • replication and transcription |
ISBN-10 | 1-4939-3039-7 / 1493930397 |
ISBN-13 | 978-1-4939-3039-5 / 9781493930395 |
Zustand | Neuware |
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