Fundamentals of Light Microscopy and Electronic Imaging (eBook)

DOUGLAS B. MURPHY supervises core facilities in microscopy and histology at the new HHMI Janelia Farm Research Campus in Ashburn, Virginia. An Adjunct Professor of Cell Biology at Johns Hopkins School of Medicine in Baltimore, Maryland, Dr. Murphy helped establish the School of Medicine Microscope Facility there, which he supervised until 2006. MICHAEL W. DAVIDSON is an assistant scholar/scientist affiliated with the National High Magnetic Field Laboratory and the Department of Biological Science at Florida State University where he is involved in developing educational websites. His digital images and photomicrographs have graced the covers of over 2,000 publications.

Chapter 1: Fundamentals of Light Microscopy

Chapter 2: Light and Color

Chapter 3: Illuminators, Filters, and the Isolation of Specific Wavelengths

Chapter 4: Lenses and Geometrical Optics

Chapter 5: Diffraction and Interference in Image Formation

Chapter 6: Diffraction and Spatial Resolution

Chapter 7: Phase Contrast Microscopy and Darkfield Microscopy

Chapter 8: Properties of Polarized Light

Chapter 9: Polarization Microscopy

Chapter 10: Differential Interference Contrast (DIC) Microscopy and Modulation Contrast Microscopy

Chapter 11: Fluorescence Microscopy

Chapter 12: Fluorescence Imaging of Dynamic Molecular Processes

Chapter 13: Confocal Laser Scanning Microscopy

Chapter 14: Two-Photon Excitation Fluorescence Microscopy

Chapter 15: Superresolution Imaging

Chapter 16: Imaging Living Cells With The Microscope

Chapter 17: Fundamentals of Digital Imaging

Chapter 18: Digital Imaging Processing

Appendix I

Appendix II

Appendix III

Glossary

References

Index

"This should be provided to all beginning graduate
students entering microscopy labs. It describes the complicated
hardware of the system, while also explaining the physics
principles of microscopy on a simplistic level for basic
biologists. The authors achieve a perfect balance of theory and
methods." (Doody's, 15 November 2013)

"It should be particularly useful to researchers getting
started in the field of microscopy as well as seasoned
professionals. Summing Up: Highly recommended. Graduate students,
researchers/faculty, and professionals/practitioners."
(Choice, 1 October 2013)

"In summary, Fundamentals of Light Microscopy, Second
Edition is a recommended starting point for the novice in
microscopy and electronic imaging." (Journal of
Biomedical Optics, 1 February 2013)