Microbial Steroids

Current Trends and Perspectives in Microbial Bioconversions of Steroids.- Isolation of Environmental Bacteria Able to Degrade Sterols and/or Bile Acids: Determination of Cholesterol Oxidase and Several Hydroxysteroid Dehydrogenase Activities in Rhodococcus, Gordonia, and Pseudomonas putida.- Selection of Biodegrading Phytosterol Strains.- Identification and Characterization of Some Genes, Enzymes, and Metabolic Intermediates Belonging to the Bile Acid Aerobic Catabolic Pathway from Pseudomonas.- Targeted Mutagenesis of Mycobacterium Strains by Homologous Recombination.- RNA Preparation and RNA-Seq Bioinformatics for Comparative Transcriptomics.- Bidimensional Analyses of the Intra- and Extra-Cellular Proteomes of Steroid-Producer Mycobacteria.- Fungal Sterol Analyses by Gas Chromatography - Mass Spectrometry Using Different Derivatives.- Cholesterol Assay Based on Recombinant Cholesterol Oxidase, ABTS, and Horseradish Peroxidase.- Measuring the Interaction of Sterols and Steroids with Proteins by Microscale Thermophoresis.- Cultivation of Mycolicibacterium spp. Mutants in Miniaturized and High-Throughput Format to Characterize Their Growth, Phytosterol Conversion Ability, and Resistance to the Steroid Products.- β-Sitosterol Bioconversion in Small-Scale Devices: From Microtiter Plates to Microfluidic Reactors.- Biocatalysis of Steroids by Mycobacterium sp. in Aqueous and Organic Media.- Scale-Up of Phytosterols Bioconversion into Androstenedione.- Bioconversion of Phytosterols into Androstenedione by Mycolicibacterium.- Selective Microbial Conversion of DHEA into 7α-ОН-DHEA.- Production of 11α-Hydroxysteroids Derivatives by Corynebacterium glutamicum Expressing the Rhizopus oryzae Hydroxylating System.- Obtaining of 24-Norchol-4-Ene-3,22-Dione from Phytosterol with Mutants of Mycolicibacterium neoaurum.- Stabilization of Enzymes by Using Thermophiles.- Stigmasterol Removal by an Aerobic Treatment System.- Glucocorticoid Effect in Cancer Patients.