Molecular Genetics of Yeast

The yeast Saccharomyces cerevisiae is used extensively in academic research and industry as a model eukaryote. It is easy to manipulate, is very flexible, and has a great many parallels with higher organisms. This makes it ideal for studies in molecular biology, genetics, and biotechnology. This book describes current experimental procedures for working with this organism.

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This book describes and discusses experimental procedures used in a wide range of specialized topics within the genetics and molecular biology of the yeast Saccharomyces cerevisiae. Contributions are by internationally-recognized experts in particular areas and represent up-to-date methods currently in use. Topics covered include DNA isolation, cloning, and expression vectors, cosmid cloning, construction and use of cDNA libraries, plasmid shuffling and
mutant isolation, Ty insertional mutagenesis, high efficiency transformation, cell-free translation of mRNAs, Ty virus-like particles, and applications to industrial strains of yeast. Detailed protocols are clearly presented, with many additional practical tips, to assist both first-time and more experienced
experimentalists.