Nucleic Acid Crystallography

Eric Ennifar Institut de Biologie Moléculaire et Cellulaire du CNRS 15 Rue René Descartes F-67084 Strasbourg, France +33 (0)3 88 41 70 02 email: e.ennifar@ibmc-cnrs.unistra.fr.

Perspectives and Pitfalls in Nucleic Acids Crystallography.- Preparation of Short 5’-Triphosphorylated Oligoribonucleotides for Crystallographic and Biochemical Studies.- Preparation and Crystallization of Riboswitches.- In Vitro/In Vivo Production of tRNA for X-Ray Studies.- Polyacrylamide Gel Electrophoresis for Purification of Large Amounts of RNA.- Use of the U1A Protein to Facilitate Crystallization and Structure Determination of Large RNAs.- Fab Chaperone-Assisted RNA Crystallography (Fab CARC).- Generating Crystallographic Models of DNA Dodecamers from Structures of RNase H: DNA Complexes.- Isothermal Titration Calorimetry: Assisted Crystallization of RNA-Ligand Complexes.- Crystallographic Data and Model Quality.- Advanced Crystallographic Data Collection Protocols for Experimental Phasing.- Nucleic Acid Crystallography via Direct Selenium Derivatization: RNAs Modified with Se-Nucleobases.- Practical Radiation Damage Induced Phasing.- Soaking Hexammine Cations into RNA Crystals to Obtain Derivatives for Phasing Diffraction Data.- Using Molecular Replacement Phasing to Study the Structure and Function of RNA.- Helical Symmetry of Nucleic Acids: Obstacle or Help in Structure Solution?.- RNA Structure Refinement Using the ERRASER-Phenix Pipeline.- Neutron Nucleic Acid Crystallography.- Reconstitution of Functionally Active Thermus thermophilus 30S Ribosomal Subunit from Ribosomal 16S RNA and Ribosomal Proteins.- Crystallographic Studies of the Ribosomal A-Site Molecular Switches by Using Model RNA Oligomers.- Structure of the HCV Internal Ribosome Entry Site Subdomain IIa RNA in Complex with a Viral Translation Inhibitor.- Anions in Nucleic Acid Crystallography.